A comparative study in five laboratories on detection ofClavibacter michiganensis subsp.sepedonicus in potato stems and tubers |
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| Authors: | S H De Boer J Van Vaerenbergh D E Stead J D Janse A R McKenzie |
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| Institution: | (1) Research Station, Agriculture Canada, 6660 N. W. Marine Drive, V6T 1X2 Vancouver, B. C., Canada;(2) Rijksstation voor Plantenziekten, Burgemeester van Gansberghelaan 96, 9820 Merelbeke, Belgium;(3) Central Science Laboratory, Ministry of Agriculture, Fisheries, and Food, AL5 2BD Hatching Green, Herts, England;(4) Plantenziektenkundige Dienst, Postbus 9102, 6700 HC Wageningen, The Netherlands;(5) Plant Protection Laboratory, A.D.R.I., Station H, P.O. Box 11300, K2H 8P9 Nepean, Ontario, Canada |
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| Abstract: | Summary Potato stems and tubers grown in the field from seed tubers inoculated withClavibacter michiganensis subsp.sepedonicus, which causes bacterial ring rot, were tested by indirect, sandwich enzyme-linked immunosorbent assay (ELISA) in five laboratories.
Correlation between values for each experimental treatment from the five laboratories was greater (r=0.86) than correlation between values for individual samples (r=0.71). When three or more laboratories obtained ELISA values of ≥0.200 for a sample, that sample was presumed to be positive.
Conversely, when three or more laboratories obtained ELISA values <0.200, the consensus determination was regarded as negative.
The percentage of stem and tuber samples that were in agreement with the consensus ELISA determination varied from 65.5 to
96.7%. Indirect immunofluorescence tests, conducted on the same samples in two laboratories, were consistent with 83.4–91.9%
of the consensus ELISA determinations. Presence or absence ofC.m. sepedonicus was confirmed in some samples by an eggplant bioassay and direct isolatiion of the bacterium. The ELISA procedure was well
suited for screening large numbers of samples and this study confirms it to be a promising procedure in routine indexing of
seed potatoes forC.m. sepedonicus. |
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| Keywords: | ring rot Solanum tuberosum L ELISA immunofluorescence eggplant detection |
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