Effects of an intravenous injection of NPY on leptin and NPY-Y1 receptor mRNA expression in ovine adipose tissue |
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| Institution: | 1. Department of Animal Science, University of Missouri, Columbia, MO 65211, USA;2. Animal Physiology Research Unit, Agricultural Research Service, USDA, Columbia, MO 65211, USA;1. Metabolism & Neurophysiology Research Group, BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea;2. SMEs Support Center, Korea Institute of Science and Technology Information (KISTI), 245 Daehangno, Yuseong-gu, Daejeon, 305-806, Republic of Korea;3. School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu, 702-701, Republic of Korea;4. Department of Functional Genomics, University of Science and Technology (UST), Daejeon 305-600, Republic of Korea;1. Department of Pharmacy, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, 200127, China;2. Department of Critical Care Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, 200127, China;3. Department of Pharmacy, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shanxi, China;1. Laboratory of Biotechnology, Chulabhorn Research Institute, 54 Kamphaeng Phet 6 Road, Lak Si, Bangkok 10210, Thailand;2. Applied Biological Science Program, Chulabhorn Graduate Institute, 54 Kamphaeng Phet 6 Road, Lak Si, Bangkok 10210, Thailand;3. Department of Biotechnology, Faculty of Science, Mahidol University, 272 Rama VI Road, Ratchathewi District, Bangkok 10400, Thailand;4. Center for Environmental Health, Toxicology and Management of Chemicals, Bangkok, Thailand;1. Douglas Mental Health University Institute, Department of Psychiatry, McGill University, Montréal, QC, Canada H4H 1R3;2. L’Oréal Research and Innovation, Clichy, France |
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| Abstract: | Neuropeptide Y (NPY) is highly expressed in hypothalami of undernourished and genetically obese animals, and is a potent regulator of food intake and reproduction. Leptin, a protein expressed by adipocytes, has been reported to reduce hypothalamic NPY expression. We recently detected (by ribonuclease protection assay RPA]) expression of the NPY receptor subtype Y1 (but not Y2) mRNA in adipose tissue. Based on these observations we hypothesized that NPY-Y1 receptors in adipose may represent a peripheral mechanism by which NPY can regulate leptin expression in a direct and rapid manner. To test this hypothesis, adipose samples were biopsied from the tailhead region of 48 ± 3 kg wether lambs immediately before and 30 min after a single intravenous injection of 50 μg porcine NPY (“treated” animals, n = 5), or vehicle (“control” animals, n = 4). Injection of NPY resulted in an increase in expression (P = 0.013; as measured by RPA) of both leptin and NPY-Y1 mRNA. In treated animals, negative correlations were found between response in leptin expression and body weight (r = −0.82, P = 0.092), and between leptin response and initial leptin mRNA levels (r = −0.81, P = 0.097). These data provide evidence of a peripheral mechanism by which NPY may regulate adipocyte expression of both leptin and NPY-Y1 receptor mRNA. |
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