| 枯草芽孢杆菌B47高产拮抗物质菌株的紫外诱变选育 |
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| 引用本文： | 王静,朱建华,林纬,黄永禄,袁高庆,黎起秦.枯草芽孢杆菌B47高产拮抗物质菌株的紫外诱变选育[J].安徽农业科学,2008,36(33). |
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| 作者姓名： | 王静 朱建华 林纬 黄永禄 袁高庆 黎起秦 |
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| 作者单位： | 广西大学农学院,广西南宁,530005 |
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| 基金项目： | 广西壮族自治区自然科学基金资助项目
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| 摘 要： | 目的]为选育对西瓜枯萎病病菌具更强抑制作用的新菌株。方法]对从番茄茎内分离获得的内生枯草芽孢杆菌B47菌株进行连续2次紫外线诱变,并对诱变菌株的遗传稳定性和生理生化特性进行测定。结果]筛选获得3株抑菌活性高于B47菌株的诱变菌株F303、F304、F305,经10代传代培养后,3株诱变菌株对西瓜枯萎病病菌的抑菌活性都有所下降,但F305的抑菌活性下降最小,遗传稳定性最好,在同等条件下其抑菌圈直径仍比野生菌株B47大5 mm。诱变菌株F305在36 h内处于对数生长期,在36~96 h内处于稳定期;最适宜生长温度为35℃;在浓度为1%~10%的钠盐中皆能生长,但以浓度为1%时生长最好,该菌株的生理生化反应与野生菌株B47的反应一致。结论]该研究为利用B47菌株进行工厂化生产拮抗性物质产品和探讨防治西瓜枯萎病的新方法奠定了基础。
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| 关 键 词： | 枯草芽孢杆菌 B47菌株 紫外线诱变 西瓜枯萎病病菌 |
Screening for the Strain Highly Producing Antagonistic Substance from Bacillus subtilis B47 by UV Mutageneisis |
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| Abstract: | Objective] The aim of this study was to breed new strains which have higher inhibitory effects on the pathogens of watermelon fusarium wilt.Method] The endophytic Bacillus subtilis B47 strain was obtained from tomato stems by UV mutagenesis for two consecutive times,then genetic stability as well as physiological and biochemical characteristics of mutant strains were studied.Result] The antibacterial activity of three mutant strains F303,F304 and F305 was higher than that of B47 strain.After subculture of 10 successive generations,the antibacterial activity of three mutant strains for the pathogens of watermelon fusarium wilt all decreased,but the antibacterial activity of F305 strain decreased the least,indicating its best genetic staility among the tested strains.The antibacterial circle diameter of F305 strain was 5 mm larger than that of wild strain B47 under the same condition.The mutant strain F305 was in logarithmic growth phase within 36 h and in stationary phase within 36-96 h,while its optimum growth temperature was 35 ℃.F305 strain could grow in sodium salt with the concentration of 1%-10%,but it grew best at the concentration of 1%.Physiological and biochemical responses of F305 strain were in accordance with those of wild strain B47.Conclusion] This study lays the foundation for the factorial production of antagonistic substance by B47 strain and new methods of preventing from the pathogens of watermelon fusarium wilt. |
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| Keywords: | Bacillus subtilis B47 strain UV mutagenesis Pathogens of watermelon fusarium wilt |
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