| 伞滑刃线虫属部分种群28S RNA基因中D2-D3区的特征 |
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| 引用本文： | 蒋立琴,郑经武.伞滑刃线虫属部分种群28S RNA基因中D2-D3区的特征[J].植物病理学报,2007,37(6):588-594. |
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| 作者姓名： | 蒋立琴 郑经武 |
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| 作者单位： | 浙江大学农业与生物技术学院生物技术研究所, 杭州 310029 |
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| 摘 要： | 对伞滑刃线虫属部分种群包括松材线虫、拟松材线虫、豆伞滑刃线虫、莱奴尔夫伞滑刃线虫和泰国伞滑刃线虫等种的28S RNA基因中D2-D3区进行了系统的研究。经PCR扩增发现, 不同的种均产生1个约750 bp的片段。利用限制性内切酶对其进行PCR-RFLP分析, 除松材线虫与拟松材线虫的酶切图谱比较接近外, 其它伞滑刃线虫种群获得不同的酶切图谱。图谱分析发现, 限制性内切酶Bsh1236I能区分2个近似种——松材线虫和拟松材线虫。进一步分析表明:Bsh1236I对28S RNA基因D2-D3区的酶切可用于鉴定本研究中伞滑刃线虫属的不同种群。同时运用clustalx1.8和Mega 2软件对28S RNA基因D2-D3区构建部分伞滑刃线虫属种群的系统树, 显示出与形态学基本一致的聚类组群。
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| 关 键 词： | 伞滑刃线虫属 28SRNA基因 PCR-RFLP 序列分析 系统学 |
| 文章编号： | 0412-0914（2007）06-0588-07 |
| 收稿时间： | 2006-09-19 |
| 修稿时间： | 2007-05-14 |
Characterization of the D2-D3 expansion regions of 28S RNA gene in some Bursaphelenchus species |
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| JIANG Li-qin,ZHENG Jing-wu.Characterization of the D2-D3 expansion regions of 28S RNA gene in some Bursaphelenchus species[J].Acta Phytopathologica Sinica,2007,37(6):588-594. |
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| Authors: | JIANG Li-qin ZHENG Jing-wu |
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| Institution: | Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China |
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| Abstract: | The possibility of 28S RNA gene on the classfication and identification of Bursaphelenchus species was studied.It was found that there was no distinct difference on the length of D2-D3 expansion regions of 28S RNA gene among different Bursaphelenchus species,all of which yield a fragment with the length of 750 bp.The PCR-RFLP patterns showed distinct differences among 3 Bursaphelenchus species,B.doui,B.rainulfi,and B.thailandae,but B.xylophilus and B.mucronatus had similar digestion patterns.One restriction enzyme,Bsh1236 I,could differentiate B.xylophilus and B.mucronatus.Further studies confirmed that all the species and populations of Bursaphelenchus and Ektaphelenchoides used in the study were differentiated by digestion of D2-D3 expansion regions of 28S RNA gene with Bsh1236 I.The phylogenetic tree were constructed according to the 12 sequences of D2-D3 expansion regions of 28S RNA gene of Bursaphelenchus species obtained from this study and GenBank,and the results corresponded well with the morphologic analysis. |
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| Keywords: | PCR-RFLP |
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