| 适应无血清培养的HEK293细胞系的培育 |
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| 引用本文： | 孟其麟,孙元,李红梅,王楠,田大勇,仇华吉.适应无血清培养的HEK293细胞系的培育[J].中国预防兽医学报,2012,34(5):375-378. |
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| 作者姓名： | 孟其麟 孙元 李红梅 王楠 田大勇 仇华吉 |
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| 作者单位： | 1. 中国农业科学院哈尔滨兽医研究所 兽医生物技术国家重点实验室/猪传染病研究室,黑龙江哈尔滨150001;延边大学农学院动物医学系,吉林延吉130001
2. 中国农业科学院哈尔滨兽医研究所 兽医生物技术国家重点实验室/猪传染病研究室,黑龙江哈尔滨,150001 |
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| 摘 要： | 为培育适合腺病毒增殖的无血清培养细胞系,本研究采用培养液渐进替换的方法获得了一株适应无血清培养的HEK293细胞(293SF),经检测293SF细胞具有稳定的支持腺病毒增殖与表达外源蛋白的能力;平均病变时间为97 h,比HEK293细胞缩短12.9%;毒价达到107.48TCID50/mL,比HEK293细胞提高43.3%;并且稳定性良好,连续传16代后293SF细胞状态与易感性未发生变化。该细胞系为腺病毒的无血清培养奠定基础。
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| 关 键 词： | HEK293细胞 无血清培养 腺病毒 |
Establishment of an HEK293 cell line adapted to serum-free culture |
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| MENG Qi-lin
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SUN Yuan
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LI Hong-mei
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WANG Nan
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TIAN Da-yong
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QIU Hua-ji.Establishment of an HEK293 cell line adapted to serum-free culture[J].Chinese Journal of Preventive Veterinary Medicine,2012,34(5):375-378. |
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| Authors: | MENG Qi-lin SUN Yuan LI Hong-mei WANG Nan TIAN Da-yong QIU Hua-ji |
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| Institution: | 1(1.Division of Swine Infectious Diseases,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences,Harbin 150001,China; 2.Department of Veterinary Medicine,Agricultural College of Yanbian University,Yanji 130001,China) |
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| Abstract: | To establish an HEK293 cell line adapted to serum-free culture,the serum-free cell line(293SF) was obtained from HEK293 cells by a gradual replacement of the cell culture medium.The 293SF and the original HEK293 were compared for the ability of adenovirus replication from passage 1 to 16.The results showed that adenovirus titer was 43.3% higher replicated in 293SF than that of in HEK293 cells and even 12.9% lesser in culture time than the original one.More importantly,the stability of 293SF was steady during the 16 passages.The new cell line could be used for large-scale production of adenovirus-based vaccines. |
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| Keywords: | HEK293 cells serum-free culture recombinant adenovirus |
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