|
|||||
|
|
| 猪捷申病毒Swine/CH/IMH/03株免疫原性的研究 | |
| 引用本文： | 冯力,时洪艳,童光志,刘娣.猪捷申病毒Swine/CH/IMH/03株免疫原性的研究[J].中国预防兽医学报,2012,34(8):655-657. |
| 作者姓名： | 冯力 时洪艳 童光志 刘娣 |
| 作者单位： | 1. 黑龙江省农业科学院博士后科研工作站东北林业大学博士后科研流动站,黑龙江哈尔滨150030;中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨150001 2. 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨,150001 3. 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨150001;中国农业科学院上海兽医研究所,上海200241 4. 黑龙江省农业科学院博士后科研工作站东北林业大学博士后科研流动站,黑龙江哈尔滨,150030 |
| 摘 要： | 为确定猪捷申病毒(Porcine Teschovirus,PTV)Swine/CH/IMH/03株免疫原性,本研究将该PTV分离株灭活后分别与氢氧化铝佐剂和Montanide ISA 50V2佐剂混合,制成油佐剂疫苗,并采用不同免疫程序接种实验猪后对病毒抗体进行检测。抗体检测结果表明:PTV Swine/CH/IMH/03分离株能够诱导实验动物产生较高抗体水平,并显著高于对照组;与氢氧化铝佐剂混合制成的佐剂疫苗免疫效果优于Montanide ISA 50V2佐剂;而且一次免疫组与二次免疫组没有明显差异。通过PTV Swine/CH/IMH/03株免疫原性的研究,为后续猪捷申病的预防工作提供技术支持。 |
| 关 键 词： | 猪捷申病毒 免疫原性研究 |
Immunogenicity of porcine teschovirus Swine/CH/IMH/03 |
|
| FENG Li , SHI Hong-yan , TONG Guang-zhi , LIU Di.Immunogenicity of porcine teschovirus Swine/CH/IMH/03[J].Chinese Journal of Preventive Veterinary Medicine,2012,34(8):655-657. | |
| Authors: | FENG Li SHI Hong-yan TONG Guang-zhi LIU Di |
| Institution: | 1(1.Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme,Northeast Forestry University Postdoctoral Programme, Harbin 150001,China;2.State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute, Chinese Academy of Agricultural Science,Harbin 150001,China; 3.Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Science,Shanghai 200241,China) |
| Abstract: | To investigate the immunogenicity of porcine Teschovirus(PTV) Swine/CH/IMH/03 isolate,the virus isolate was inactivated and emulsified with aluminum hydroxide or Montanide ISA 50V2 adjuvant to prepare the oil adjuvant vaccines.The antibodies against PTV were detected in piglets immunized with the oil adjuvant vaccines once or twice via intramuscular injection,respectively.Antibody titrations results showed that inactivated PTV Swine/CH/IMH/03 was able to efficiently induce antibody response in experimental animals.The antibodies of immunized group were significantly higher than of unimmunized group.Immunization of inactivated PTV emulsified with aluminum adjuvant had a better effect than that of Montanide ISA 50V2 adjuvant.However,it was no significant difference for once or twice immunization.In conclusion,the study of PTV Swine/CH/IMH/03 immunogenicity provides technical support for porcine teschen disease prevention. |
| Keywords: | porcine teschovirus immunogenicity |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |