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| 水貂犬瘟热CDV_3疫苗株基因组序列测定及H基因遗传稳定性分析 | |
| 引用本文： | 赵建军,柴秀丽,王凤雪,邵西群,陈涛,罗国良,闫喜军,吴威.水貂犬瘟热CDV_3疫苗株基因组序列测定及H基因遗传稳定性分析[J].经济动物学报,2009,13(1). |
| 作者姓名： | 赵建军 柴秀丽 王凤雪 邵西群 陈涛 罗国良 闫喜军 吴威 |
| 作者单位： | 1. 中国农业科学院特产研究所人兽共患病研究室,吉林,132109 2. 吉林农业大学动物科技学院,长春,130118 3. 中国农业科学院特产研究所人兽共患病研究室,吉林,132109;吉林中特生物技术有限公司,吉林,132109 |
| 基金项目： | 科技部科研院所社会公益研究专项基金 |
| 摘 要： | 对水貂犬瘟热CDV3疫苗株基因组进行全序列测定与分析,以阐明该疫苗株的来源亲本毒株,基因特征及H基因遗传稳定性。将CDV3疫苗株基因组cDNA分9个重叠片段进行RT-PCR并克隆入pMD 18-T载体中,测定全长cDNA序列。并与CDV野毒参考株和疫苗株N、F和H基因氨基酸序列比对分析其基因变异情况。通过对CDV3疫苗株6个不同生产批次(Vero细胞培养代次)H基因序列测定以分析毒株的分子遗传稳定性。基因序列分析结果表明:CDV3疫苗株基因组全长15 690 bp,与CDV野毒株基因组同源性较低(93.0%~95.3%)。H基因核苷酸和氨基酸序列与Lederle疫苗株(EF418783)同源性最高,分别为99.6%和98.7%。而6个不同培养代次CDV3疫苗株H基因氨基酸序列无任何差异。由此证实CDV3疫苗株的亲本毒株与Lederle疫苗株有着最密切关系,不同培养代次的CDV3疫苗株H基因具有较高的遗传稳定性。 |
| 关 键 词： | 犬瘟热病毒 CDV3疫苗株 基因组序列 遗传稳定性 |
Genomic Sequence Determination and Genetic Stability of Minks Canine Distemper Virus Vaccine CDV_3 Strain |
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| ZHAO Jian-jun,CHAI Xiu-li,WANG Feng-xue,SHAO Xi-qun,CHEN Tao,LUO Guo-liang,YAN Xi-jun,WU Wei.Genomic Sequence Determination and Genetic Stability of Minks Canine Distemper Virus Vaccine CDV_3 Strain[J].Journal of Economic Animal,2009,13(1). | |
| Authors: | ZHAO Jian-jun CHAI Xiu-li WANG Feng-xue SHAO Xi-qun CHEN Tao LUO Guo-liang YAN Xi-jun WU Wei |
| Institution: | 1.Division of Zoonosis;Institute of Special Economic Animal and Plant Sciences;Chinese Academy of Agricultural Sciences;Jilin 132109;China;2.Jilin Zhongte Biotechnological Co.Ltd;3.College of Animal Science and Technology;Jilin Agricultural University;Changchun 130118;China |
| Abstract: | To determine the genomic sequence and evaluate the genetic stability of canine distemper virus vaccine(CDV) CDV3 strain,nine cDNA fragments covering the complete genome of the strain,and its complete genomic sequence was assembled and analyzed..The H genes of six generations of CDV3 strain cultured with Vero cell were cloned and sequenced.The genome of CDV3 strain consists of 15 690 nucleotides and homology showed 93.0%-95.3% identities at nucleotide level in comparison of the sequence of CDV3 strain with t... |
| Keywords: | canine distemper virus CDV3 vaccine strain genomic sequence genetic stability |
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