Analysis of a single nucleotide polymorphism that controls the cooking quality of rice using a non-gel based assay |
| |
| Authors: | Concetta A Bormans Richard B Rhodes Daniel D Kephart Anna M McClung William D Park |
| |
| Institution: | (1) Borlaug Center for Southern Crop Improvement, Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX, 77843, U.S.A;(2) Promega Corporation, 2800 Woods Hollow Road, Madison, WI, 53711, U.S.A;(3) USDA-ARS, 1509 Aggie Dr., Beaumont, TX, 77713, U.S.A |
| |
| Abstract: | The waxy gene encoding granule-bound starch synthase (GBSS) is responsible for the synthesis of amylose in developing grain.
Recent work has shown that a G-T polymorphism in the leader intron 5' splice site of GBSS plays a key role in determining
the cooking and processing quality of rice. Cultivars with sequence AGGTATA at this location splice GBSS pre-mRNA efficiently
and produce relatively large amounts of amylose. These varieties generally a have firm texture when cooked and the grains
remain separate. In contrast, GBSS pre-mRNA splicing is temperature sensitive and generally less efficient in cultivars with
the sequence AGTTATA. As a result, these cultivars generally have lower amylose content and produce soft and sticky cooked
rice. We have used the READITTM assay, anon-gel based assay that uses the ability of DNA polymerase to perform pyrophosphoralysis, the reverse of DNA polymerization,
to screen the critical G-T polymorphism in more than 750 samples from U.S. and Asian germplasm. We observed complete concordance
between the results obtained using DNA sequencing or restriction enzyme digestion and the READITTM assay. It also gave accurate results with both heterozygous plants and with complex mixtures as might result when grain from
advanced generation plants is pooled to obtain larger samples.
This revised version was published online in August 2006 with corrections to the Cover Date. |
| |
| Keywords: | amylose granule-bound starch synthase READITTM assay single-nucleotide polymorphism waxy gene |
| 本文献已被 SpringerLink 等数据库收录！ |
|
