| 广东地区患病食品动物源大肠杆菌ESBLs和AmpC酶流行分布调查 |
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| 引用本文： | 汤电,张浩吉,纪雪薇,刘继,郭玉芳,王丽华,付晓平,张小华,孙永学,蒋红霞.广东地区患病食品动物源大肠杆菌ESBLs和AmpC酶流行分布调查[J].中国兽医学报,2012,32(2):242-247. |
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| 作者姓名： | 汤电 张浩吉 纪雪薇 刘继 郭玉芳 王丽华 付晓平 张小华 孙永学 蒋红霞 |
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| 作者单位： | 1. 华南农业大学兽医学院广东省兽药研制与安全评价重点实验室,广东广州,510642
2. 佛山科学技术学院生命科学学院,广东佛山,528231 |
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| 基金项目： | 国家自然科学基金资助项目(30871890);国家自然科学基金-广东省自然科学基金联合基金重点资助项目(U0631006) |
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| 摘 要： | 质粒介导的超广谱β-内酰胺酶（ESBLs）和AmpC酶广泛传播和扩散,导致全球范围内肠杆菌科细菌对超广谱头孢菌素类的耐药性发展迅速,引起人们高度重视。食品动物源大肠杆菌可作为耐药基因储库,对细菌耐药性在动物、环境和人之间的传播起着非常重要的作用。本研究从2009年患病的食品动物,包括鸡、鸭、鹅和猪,分离鉴定了315株大肠杆菌,调查ESBLs和AmpC酶在患病食品动物中的流行分布。经双纸片协同扩散实验筛选出61株ESBL阳性菌株,PCR检测共检测出8种blaCTX-M基因,分别为blaCTX-M-14/14b、blaCTX-M-79、blaCTX-M-65、blaCTX-M-27、blaCTX-M-15、blaCTX-M-24、blaCTX-M-98和blaCTX-M-13,其中检出率最高的为blaCTX-M-79,其次为blaCTX-M-14/14b。本研究还检测出1株新的SHV型酶,命名为SHV-135;PCR共检测出5株大肠杆菌携带质粒源CMY-型AmpC酶,其中2株鹅源大肠杆菌携带1个新型CMY酶,命名为CMY-64。本研究表明患病动物分离的大肠杆菌中ESBLs和AmpC酶存在复杂性和多样性,而且新型β-内酰胺酶检出日益增多,提示兽医临床应慎用β-内酰胺类抗生素。
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| 关 键 词： | 食品动物源大肠杆菌 耐药性 ESBLs AmpC酶 质粒 |
Prevalence of plasmid mediated ESBLs and AmpC-type β-lactamases among E.coli isolated from sick food-producing animals in Guangdong |
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| TANG Dian,ZHANG Hao-ji,JI Xue-wei,LIU Ji,GUO Yu-fang,WANG Li-hua,FU Xiao-ping,ZHANG Xiao-hua,SUN Yong-xue,JIANG Hong-xia.Prevalence of plasmid mediated ESBLs and AmpC-type β-lactamases among E.coli isolated from sick food-producing animals in Guangdong[J].Chinese Journal of Veterinary Science,2012,32(2):242-247. |
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| Authors: | TANG Dian ZHANG Hao-ji JI Xue-wei LIU Ji GUO Yu-fang WANG Li-hua FU Xiao-ping ZHANG Xiao-hua SUN Yong-xue JIANG Hong-xia |
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| Institution: | 1*(1.College of Veterinary Medicine,Guangdong Key Laboratory for Veterinary Drug Development and Safety Evaluation,South China Agricultural University,Guangzhou 510642,China;2.College of Life Science,Foshan Science and Technology University,Foshan,Guangdong 528231,China) |
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| Abstract: | Extensive dissemination and spread of plasmid mediated extended spectrum β-lactamases(ESBLs) and AmpC-type β-lactamases resulted in a rapid development of resistance to extended spectrum cephalosporins in Enterobacteriaceae worldwide,causing globe concerns.Food-producing animal origin E.coli,as a resistant gene reserviors,plays an important role in resistance dissemination among animals,environment and human.The present study isolated and identified 315 E.coli from sick food-producing animals,including chickens,ducks,geese and pigs,investigated the prevalence of ESBLs and AmpC among sick food-producing animals.Sixty-one ESBLs positive E.coli were selected by double disc synergy test.Eight varieties blaCTX-M-type genes in total were detected,including blaCTX-M-14/14b、blaCTX-M-79、blaCTX-M-65、blaCTX-M-27、 blaCTX-M-15、blaCTX-M-24、blaCTX-M-98 and blaCTX-M-13.A novel SHV-type ESBLs was identified and assigned as SHV-135 in a duck origin E.coli.CMY-type AmpC β-lactamases weredetected in 5 E.coli,3 of which was CMY-2,and 2 of which were novel AmpC enzyme and had been assigned as CMY-64.The present study showed that the prevalence of ESBLs and AmpC complex and diversity among sick food-producing animals origin E.coli,the detection of ESBLs and AmpC was increasing,suggesting prude use of β-lactams in veterinary clinic. |
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| Keywords: | food-producing animal origin E coli resistance ESBLs AmpC enzyme plasmid |
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