| 鸡Lpin1基因3′-UTR遗传变异及其对miRNA结合位点的潜在效应 |
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| 引用本文： | 李素雅,张建宏,陈文,黄艳群,孙桂荣,韩瑞丽,康相涛.鸡Lpin1基因3′-UTR遗传变异及其对miRNA结合位点的潜在效应[J].中国农业科学,2012,45(8):1613-1620. |
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| 作者姓名： | 李素雅 张建宏 陈文 黄艳群 孙桂荣 韩瑞丽 康相涛 |
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| 作者单位： | 1.河南农业大学生命科学学院，郑州 450002;
2.河南农业大学牧医工程学院/家禽种质资源创新工程中心，郑州 450002 |
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| 基金项目： | 国家自然科学基金(30771533);郑州市创新型科技人才队伍建设工程科技创新(096SYJH16092);家养动物种质资源平台 |
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| 摘 要： | 【目的】研究鸡Lpin1基因3′-UTR遗传变异/单倍型及其在品种间的分布，并预测这些变异对miRNA结合位点的潜在效应。【方法】根据鸡Lpin1基因组序列（GenBank登录号：NC_006090）设计一对特异性引物，采用PCR直接测序结合克隆测序的方法，进行鸡Lpin1基因3′-UTR及其邻近外显子在品种间的遗传变异/单倍型分析。【结果】①从6个品种中发现了8个变异位点，包含两个插入/缺失变异。这些变异均位于鸡Lpin1基因的3′-UTR，3′-UTR的变异频率为17SNPs/kb；②在鸡Lpin1基因3′-UTR区域，从固始鸡、卢氏绿壳蛋鸡中分别检测到7个变异位点，未检测到河南斗鸡品种内的变异；③用次要等位基因频率≥5%的6个变异位点（g.11A＞T，g.77C＞G，g.108_109delinsG，g.110_111delinsG，g.270A＞G和g.348G＞T）进行单倍型的构建和分析，从6个品种鸡中共检测到6种单倍型，其中P1和P4单倍型是群体的优势单倍型，频率均大于30%，河南斗鸡仅包含一种单倍型；④g.77C＞G变异可引起多个miRNA结合位点的增加/丢失。【结论】鸡Lpin1基因3′-UTR具有丰富的变异，3′-UTR变异位点/单倍型在品种间的分布存在明显的差异。
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| 关 键 词： | 鸡 Lpin1基因 3′-UTR 遗传变异 miRNA靶位点 |
| 收稿时间： | 2011-06-02 |
Genetic Variation Analysis of 3′-UTR Region of Chicken Lpin1 Gene and the Potential Effect on miRNA Binding Sites |
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| LI Su-ya,ZHANG Jian-hong,CHEN Wen,HUANG Yan-qun,SUN Gui-rong,HAN Rui-li,KANG Xiang-tao.Genetic Variation Analysis of 3′-UTR Region of Chicken Lpin1 Gene and the Potential Effect on miRNA Binding Sites[J].Scientia Agricultura Sinica,2012,45(8):1613-1620. |
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| Authors: | LI Su-ya ZHANG Jian-hong CHEN Wen HUANG Yan-qun SUN Gui-rong HAN Rui-li KANG Xiang-tao |
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| Institution: | 1College of Life Sciences,Henan Agricultural University,Zhengzhou 450002;2College of Animal Science and Veterinary Medicine,Henan Agricultural University/Henan Research Center of Breeding Resources for Poultry,Zhengzhou 450002) |
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| Abstract: | 【Objective】 This experiment was conducted to study the genetic variation/haplotype of the 3′-UTR of chicken Lpin1 gene and their distributions among breeds,and their potential effects on miRNA binding sites were predicted.【Method】 According to the chicken Lpin1 genome sequence（GenBank accession: NC006090）,a pair of specific primers was designed to conduct the genetic variation/haplotype analysis in the 3′-UTR of chicken Lpin1 gene in different breeds of chickens through direct PCR sequence combined with clone sequencing.【Result】Eight variable sites were uncovered,these variation sites were all located at the 3′-UTR of chicken Lpin1 gene.Analysis showed the no variation sites were detected from Henan Game chicken in this region,however,six variation sites were detected from Gushi and Lushi chickens.Six variable sites（g.11A> T,g.77C > G,g.108109delinsG,g.110111delinsG,g.270A>G and g.348G>T） with minor allele frequency ≥5% were selected for haplotype reconstruction,and six haplotypes were detected from six chicken breeds.P1 and P4 were the predominant haplotypes with frequencies >30%,respectively,only one haplotype was detected from Henan game chicken.Software prediction showed that g.77C>G variant could cause the increase/loss of several miRNA binding sites.【Conclusion】 The results showed that the 3′-UTR of chicken Lpin1 gene was highly polymorphic,the distribution of variable sites and haplotypes in different breeds of chickens had clear difference. |
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| Keywords: | chicken Lpin1 gene 3′-UTR genetic variation miRNA target site |
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