| 鸡柔嫩艾美耳球虫不同抗药性虫株的种内多态性研究 |
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| 引用本文： | 于咏兰,汪明.鸡柔嫩艾美耳球虫不同抗药性虫株的种内多态性研究[J].畜牧兽医学报,2001,32(6):544-548. |
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| 作者姓名： | 于咏兰 汪明 |
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| 作者单位： | 中国农业大学动物医学院, |
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| 摘 要： | 应用RAPD技术进行柔嫩艾美耳球虫4个单一抗药性虫株与1个敏感株的基因组DNA多态性分析，发现4个抗药性虫株及敏感株之间的相似值均大于99%；OPAO3引物对抗盐霉素株扩增出一条约500bp的特异条带，OPH02引物对抗克球粉株和抗盐霉素株均扩增出了约1kb的第三条主带，这些特异条带的出现很可能与球虫抗药性基因有关，有可能用于抗药性虫株的诊断与鉴定。
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| 关 键 词： | 柔嫩艾美耳球虫 抗药性 RAPD 虫株 多态性 鸡病 |
| 文章编号： | 0366-6964(2001)06-0544-05 |
STUDY OF INTRASPECIFIC POLYMORPHISMS OF EIMERIA TENELLADUE TO RESISTANCE AGAINST ANTICOCCIDIAL DRUGS |
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| YU Yong lan,WANG Ming.STUDY OF INTRASPECIFIC POLYMORPHISMS OF EIMERIA TENELLADUE TO RESISTANCE AGAINST ANTICOCCIDIAL DRUGS[J].Acta Veterinaria et Zootechnica Sinica,2001,32(6):544-548. |
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| Authors: | YU Yong lan WANG Ming |
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| Abstract: | The genomic DNA of 4 single drug resistant strains and 1 sensitive strain of Eimeria tenella were analysed by RAPD(Random Amplified Polymorphic DNA),the results demonstrated that the similarity coefficients(SI) between all strains were over 99 0%;RAPD-PCR fingerprints generated with primer 5' AGTCAGCCAC 3'(OPA03)revealed that one band with a molecular size of approximately 500 bp was present in the DNA template of the salinomycin resistant strain but were undetectable in the other four strains;RAPD PCR fingerprints of the salinomycin resistant strain and the clopidol resistant strain with primer 5' TCGGACGTGA 3'(OPH02)appeared the same polymorphic fragment around 1kb and this wasn't the case in the other three strains.These polymorphic fragments might be due to the existance of coccidial drug resistant genes and might lead to the design of diagnostic testes for Eimeria resistant genotypes. |
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| Keywords: | Eimeria tenella Drug resistance RAPD |
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