| 玉米耐旱相关基因dbf1的序列变异分析 |
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| 引用本文： | 杨泽峰,张恩盈,蒋莹,徐扬,潘亮,陈庆,徐辰武.玉米耐旱相关基因dbf1的序列变异分析[J].玉米科学,2014,22(5):20-28. |
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| 作者姓名： | 杨泽峰 张恩盈 蒋莹 徐扬 潘亮 陈庆 徐辰武 |
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| 作者单位： | 扬州大学农学院/教育部植物功能基因组学重点实验室/江苏省作物遗传生理重点实验室, 江苏扬州225009;扬州大学农学院/教育部植物功能基因组学重点实验室/江苏省作物遗传生理重点实验室, 江苏扬州225009;扬州大学农学院/教育部植物功能基因组学重点实验室/江苏省作物遗传生理重点实验室, 江苏扬州225009;扬州大学农学院/教育部植物功能基因组学重点实验室/江苏省作物遗传生理重点实验室, 江苏扬州225009;扬州大学农学院/教育部植物功能基因组学重点实验室/江苏省作物遗传生理重点实验室, 江苏扬州225009;扬州大学农学院/教育部植物功能基因组学重点实验室/江苏省作物遗传生理重点实验室, 江苏扬州225009;扬州大学农学院/教育部植物功能基因组学重点实验室/江苏省作物遗传生理重点实验室, 江苏扬州225009 |
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| 基金项目： | 高等学校博士学科点专项科研基金项目（20123250110001）、国家重点基础研究发展计划“（973 计划”）项目（2011CB100100）、国家自然科学基金项目（31371632、31200943、31171187）、江苏省自然科学基金项目（BK2012261）、江苏省“青蓝工程”科技创新团队、江苏省普通高校研究生科研创新计划项目（CXZZ12_0907） |
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| 摘 要： | 玉米dbf1 基因在干旱胁迫下的编码产物与DIP1蛋白相结合，调控植物激素脱落酸（ABA）响应基因rab17 的表达，从而提高耐旱性。以B73的dbf1 基因组序列为模板，对104个玉米自交系的dbf1 基因进行测序，研究玉米dbf1 基因的序列多态性。多态性分析表明，在全长为2 118 bp的序列中共发现48个SNP和12个InDel。尽管大部分变异位点集中在非编码区，但编码区的1个InDel和3个非同义突变位点的变异可以产生氨基酸序列改变。玉米dbf1 基因的全长序列和编码区序列的变异位点可以分别将该基因划分成33和11种单倍型，并且编码7种蛋白质。在供试材料中，dbf1 基因至少经历了9次重组，中性检测表明，该基因的进化没有偏离中性选择。
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| 关 键 词： | 玉米 dbf1 基因 单核苷酸多态性（SNP） InDel 连锁不平衡 单倍型 |
| 收稿时间： | 2013/12/30 0:00:00 |
Nucleotide Diversity of Drought Tolerance Related Maize Gene dbf1 |
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| YANG Ze-feng,ZHANG En-ying,JIANG Ying,XU Yang,PAN Liang,CHEN Qing and XU Chen-wu.Nucleotide Diversity of Drought Tolerance Related Maize Gene dbf1[J].Journal of Maize Sciences,2014,22(5):20-28. |
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| Authors: | YANG Ze-feng ZHANG En-ying JIANG Ying XU Yang PAN Liang CHEN Qing and XU Chen-wu |
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| Institution: | Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of the Ministry of Education for Plant Functional Genomics, Yangzhou University, Yangzhou 225009, China;Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of the Ministry of Education for Plant Functional Genomics, Yangzhou University, Yangzhou 225009, China;Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of the Ministry of Education for Plant Functional Genomics, Yangzhou University, Yangzhou 225009, China;Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of the Ministry of Education for Plant Functional Genomics, Yangzhou University, Yangzhou 225009, China;Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of the Ministry of Education for Plant Functional Genomics, Yangzhou University, Yangzhou 225009, China;Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of the Ministry of Education for Plant Functional Genomics, Yangzhou University, Yangzhou 225009, China;Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of the Ministry of Education for Plant Functional Genomics, Yangzhou University, Yangzhou 225009, China |
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| Abstract: | The protein product encoded by maize dbf1 gene will combine with DIP1 protein to regulate ABA responsive gene rab17 expression under drought stress, resulting in improvement of drought tolerance. In order to illustrate the sequence polymorphism of maize gene dbf1, the dbf1 gene in 104 maize inbred lines was sequenced, using the genomic sequence of B73 as the reference. A total of 48 SNPs and 12 InDels were identified among 2 118 bp nucleotide sequences at this locus. Although most of these polymorphic sites were found to be located in non-coding regions, one InDel and three nonsynonymous mutations in coding regions may change the amino acid sequences. The polymorphic sites in the genomic sequences andcoding sequences of maize dbf1 classified all the tested inbred lines into 33 and 11 haplotypes, respectively. In addition, seven deferring DBF1 proteins were encoded by these inbred lines. Analysis of the polymorphism sites revealed that at least 9 recombination events had occurred in dbf1 locus. Furthermore, the evolution of maize dbf1 gene in the tested lines fit the neutral theory model. |
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| Keywords: | Maize dbf1 gene SNP InDel Linkage disequilibrium Haplotype |
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