| 太空环境诱导的草地早熟禾皱叶突变体蛋白质组学研究 |
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| 引用本文： | 韩蕾,孙振元,巨关升,钱永强,彭镇华.太空环境诱导的草地早熟禾皱叶突变体蛋白质组学研究[J].核农学报,2005,19(6):417-420. |
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| 作者姓名： | 韩蕾 孙振元 巨关升 钱永强 彭镇华 |
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| 作者单位： | 中国林业科学研究院林业研究所/国家林业局林木培育重点实验室,北京,100091;中国林业科学研究院林业研究所/国家林业局林木培育重点实验室,北京,100091;中国林业科学研究院林业研究所/国家林业局林木培育重点实验室,北京,100091;中国林业科学研究院林业研究所/国家林业局林木培育重点实验室,北京,100091;中国林业科学研究院林业研究所/国家林业局林木培育重点实验室,北京,100091 |
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| 基金项目： | 国家“863”计划：优质抗逆草坪草早熟禾等新品种选育（2002AA241061） |
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| 摘 要： | “神舟3号”飞船搭载草地早熟禾(Poa pratensisL.)‘Nassau’干种子,地面种植后筛选出皱叶突变株系PM2。利用蛋白质组学技术对PM2突变株系叶片中表达的蛋白质进行解析(未搭载植株为对照),发现基本保持不变的蛋白质点有181个,上调节表达蛋白质点有15个,下调节表达蛋白质点有15个,失去表达蛋白质点有116个。选择19个蛋白质点进行肽质谱分析,同源性查询后其中10个蛋白质点得到阳性结果,且PM2-058为谷氨酸合成酶类似蛋白,PM2-175为磷酸丙糖异构酶类似蛋白;CK-103为ATP酶依赖型26S蛋白酶体类似蛋白;CK-330为1,5二磷酸核酮糖羧化酶/加氧酶类似蛋白;CK-376为硫氧还蛋白M前体类似蛋白;CK-391为二磷酸核酮糖羧化酶类似蛋白。
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| 关 键 词： | 太空环境 草地早熟禾 皱叶突变株系 蛋白质组学 |
| 文章编号： | 1000-8551（2005）06-417-04 |
| 收稿时间： | 2005-07-11 |
| 修稿时间： | 2005年7月11日 |
PROTEOMIC ANALYSIS OF A WINNKLE-LEAF MUTANT IN Poa pratensis L.INDUCED BY AEROSPACE ENVIRONMENT |
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| HAN Lei,SUN Zhen-yuan,JU Guan-sheng,QIAN Yong-qiang,PENG Zhen-hua.PROTEOMIC ANALYSIS OF A WINNKLE-LEAF MUTANT IN Poa pratensis L.INDUCED BY AEROSPACE ENVIRONMENT[J].Acta Agriculturae Nucleatae Sinica,2005,19(6):417-420. |
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| Authors: | HAN Lei SUN Zhen-yuan JU Guan-sheng QIAN Yong-qiang PENG Zhen-hua |
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| Institution: | Research Institute of Forestry, Chinese Academy of Forestry/Key Laboratory of Forest Cultivation, State Forestry Administration, Belting, 100091 |
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| Abstract: | The dry seeds of Poa pratensis L.variety 'Nassau' were carried by spaceship SZ-3 as treatments and the seeds which on the earth as CK.A winnkle-leaf mutant which named PM_2 was selected for the analysis.Proteins expressed in leaf of CK and PM_2 were compared and profiled with the techniques of proteomics.There are 181 proteins maintained no changes,15 proteins were up-regulated and 15 proteins were down-regulated,and 116 proteins were lost. 19 proteins were chosen to make pdypeptide fingerprint and homelogy analysis.It indicated that,10 of them were positive results and protein PM_2-058 was close to glutamine synthetase,PM_2-175 was close to triosephosphate isomerase,CK-103 was close to 26S proteasome regulatory complex ATPase,CK-330 was close to ribulose 1,5 biphosphate carboxylase/oxygenase,CK-376 was close to thioredoxin M precursor,CK-391 was close to ribulose bisphosphate carboxylase. |
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| Keywords: | aerospace condition Poa pratensis L winnkle-leaf mutant protemics |
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