| 光敏胞质不育小麦花药发育过程中ATP酶的定位研究 |
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| 引用本文： | 孟祥红,王建波,利容千.光敏胞质不育小麦花药发育过程中ATP酶的定位研究[J].作物学报,2000,26(6):851-860. |
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| 作者姓名： | 孟祥红 王建波 利容千 |
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| 作者单位： | 武汉大学植物发育生物学教育部重点实验室 湖北武汉 430072 |
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| 基金项目： | 湖北省自然科学基金，武汉大学青年都师基金资助项目 |
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| 摘 要： | 采用磷酸铅沉淀法(酶孵育离子为Mg2+)研究了不同日照条件下光敏胞质不育 小麦花药发育过程中ATPase分布的变化。 结果表明： 短日照条件下花粉可育， 单核早 期ATPase主要分布在花粉表面和细胞核中， 花粉外壁、 质膜及细胞质中有少量的ATPas e 分布； 随着花粉的发育， 其表面、 外壁、 内壁、 质膜及细胞质内ATPase进一步增
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| 关 键 词： | 光敏胞质不育 小麦 花药发育 光周期 ATPase 细胞化学 |
| 收稿时间： | 1999-10-14 |
| 修稿时间： | 1999-10-14 |
Ultrastructural Localization of ATPase Activity in Anther of Photoperiod-sensitive Cytoplasmic Male-sterile Wheat |
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| MENG Xiang Hong WANG Jian Bo,LI Rong Qian.Ultrastructural Localization of ATPase Activity in Anther of Photoperiod-sensitive Cytoplasmic Male-sterile Wheat[J].Acta Agronomica Sinica,2000,26(6):851-860. |
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| Authors: | MENG Xiang Hong WANG Jian Bo LI Rong Qian |
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| Institution: | Key laboratory of MDE for plant Developemnt Biology Wuhan University, Wuhan 430072 |
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| Abstract: | Lead phosphate was used to locate ATPase in fertile and sterile anth ers of photoperiod sensitive cytoplasmic male-sterile wheat ( Triticum aestivu m L.). The quantity and distribution of ATPase in pollen changed during the po llen development and were different between fertile and sterile anthers under differe nt daylight exposure. The anthers were fertile under short daylight exposure. AT Pase mainly distributed on the surface and nucleus of preuninucleate pollen whil e little ATPase appeared in exine,cytoplasm and on plasmalemma at the same stage . ATPase distributed on the surface of pollen,plasmalemma, in exine and cytopla sm gradually increased with the pollen development. When the pollen was about to m ature,there was little ATPase distribution on the cytoplasm of trinucleate polle n in which abundant starch grains accumulated,but abundant ATPase located on the surface of pollen, in exine, vegetative nucleus and sperm cells. The quantity and distribution of ATPase in the cells of anther including anther wall and c onnective tissue changed, which correlated with the pollen development. The anthers were sterile under long daylight exposure. The ATPase distributed in st erile anthers was different in comparison with the fertile anthers,showing that the lack of substance and energy resulted in the early pollen abortion but was n ot the direct reason for the late abortive pollen. |
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| Keywords: | Photoperiod sensitive cytoplasmic male st erility Triticum aestivum L Anther development Photoperiod ATPase Cy tochemisd |
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