| 赤霉素（GA3）和脱落酸（ABA）对不同水稻品种生长和生理特性及GA20ox2、GA3ox2基因表达的影响 |
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| 引用本文： | 杨艳华,张亚东,朱镇,赵凌,陈涛,赵庆勇,王才林.赤霉素（GA3）和脱落酸（ABA）对不同水稻品种生长和生理特性及GA20ox2、GA3ox2基因表达的影响[J].中国水稻科学,2010,24(4):433-437. |
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| 作者姓名： | 杨艳华 张亚东 朱镇 赵凌 陈涛 赵庆勇 王才林 |
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| 作者单位： | 江苏省农业科学院,粮食作物研究所/江苏省优质水稻工程技术研究中心/国家水稻改良中心南京分中心,江苏,南京,210014 |
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| 基金项目： | 江苏省人事厅博士后资助项目，江苏省农业科学院博士后基金资助项目，江苏省农业科技自主创新基金项目 |
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| 摘 要： | 以5个水稻品种4叶1心期的幼苗为材料，比较研究了1 μmol/L GA3和10 mg/L ABA处理对不同水稻品种生长发育、生理特性及基因表达的影响。结果表明，GA3处理能够促进水稻地上部分伸长生长，抑制根伸长生长；同时，5个品种叶片的叶绿素含量、可溶性蛋白质含量和细胞膜透性呈上升趋势。10 mg/L ABA处理则严重抑制种子萌发和地上部伸长生长；5个品种叶片的叶绿素含量、可溶性蛋白质含量和细胞膜透性则呈现下降趋势。 用RT PCR方法对GA20ox2和GA3ox2基因表达水平的变化进行检测，结果表明GA3处理后，日本晴GA20ox2基因表达略微下调，其余4个品种的GA20ox2基因表达上调。ABA处理抑制GA20ox2基因表达。GA3ox2基因表达也受GA3和ABA调控。在南粳44、关东194和日本晴中，GA3诱导GA3ox2表达，ABA显著抑制GA3ox2表达。性
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| 关 键 词： | 水稻 赤霉素 脱落酸 矮秆基因 基因表达 生长 生理特 |
| 收稿时间： | 1900-01-01; |
Effects of Gibberellic Acid 3 and Abscisic Acid on Growth, Physiological Characteristics and Gene Expression of GA20ox2 and GA3ox2 in Different Rice Cultivars |
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| YANG Yan-hua,ZHANG Ya-dong,ZHU Zhen,ZHAO Ling,CHEN Tao,ZHAO Qing-yong,WANG Cai-lin.Effects of Gibberellic Acid 3 and Abscisic Acid on Growth, Physiological Characteristics and Gene Expression of GA20ox2 and GA3ox2 in Different Rice Cultivars[J].Chinese Journal of Rice Science,2010,24(4):433-437. |
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| Authors: | YANG Yan-hua ZHANG Ya-dong ZHU Zhen ZHAO Ling CHEN Tao ZHAO Qing-yong WANG Cai-lin |
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| Institution: | Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Jiangsu High Quality Rice R &; D Center/Nanjing Branch of Chinese National Center for Rice Improvement, Nanjing 210014, China; *Corresponding author， E-mail: clwang@jaas.ac.cn |
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| Abstract: | Effects of exogenous gibberellic acid 3 (GA3) and abscisic acid (ABA) on the growth, physiological characteristics and gene expression of GA20ox2 and GA3ox2 were studied at four leaf stage with five rice cultivars. Results showed that 1 μmol/L GA3 could promote seedling height, restrain root elongation, and increase the contents of chlorophyll and soluble protein, and permeability of cell membrane. However, 10 mg/L ABA could inhibit the seed germination heavily and also retard seedling height and decrease the contents of chlorophyll and soluble protein, and permeability of cell membrane. RT PCR detection showed that the expression of GA20ox2 was obviously up regulated under the GA3 treatment, except for Nipponbare. On the contrary, ABA restrained the expression of GA20ox2. In the three rice cultivars, Nanjing 44, Kanto 194 and Nipponbare, GA3 induced the expression of GA3ox2 and ABA inhibited the expression of GA3ox2. |
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| Keywords: | rice gibberellic acid abscisic acid dwarf gene gene expression growth physiological traits |
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