| 双孢蘑菇褐腐病病原菌的分离及分子鉴定 |
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| 引用本文： | 李河,周国英,刘君昂.双孢蘑菇褐腐病病原菌的分离及分子鉴定[J].食用菌学报,2009,16(2). |
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| 作者姓名： | 李河 周国英 刘君昂 |
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| 作者单位： | 中南林业科技大学生物技术开放性中心实验室,中南林业科技大学林学院,湖南长沙,410004 |
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| 基金项目： | 国家林业部"948"资助项目 |
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| 摘 要： | 对引起双孢蘑菇(Agaricus bisporus)褐腐病的病原菌进行分离和分子鉴定.分离培养褐腐病病原菌,采用CTAB法抽提其基因组总DNA,利用真菌通用引物ITS1和ITS4扩增菌株rDNA ITS区序列,扩增产物纯化后克隆转化至大肠杆菌,挑选克隆成功的菌株进行测序.测序结果在GenBank中进行同源性搜索,并下载部分具有代表性种的ITS序列,利用软件MEGA4 构建分子系统发育树,通过序列分析,鉴定出引起双孢蘑菇褐腐病的病原菌为Mycogone perniciosa.
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| 关 键 词： | 双孢蘑菇 褐腐病 转录间隔区 分子鉴定 |
Isolation and Molecular Identification of a Pathogen Causing Brown Rot in Agaricus bisporus |
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| LI He,ZHOU Guoying,LIU Junang.Isolation and Molecular Identification of a Pathogen Causing Brown Rot in Agaricus bisporus[J].Acta Edulis Fungi,2009,16(2). |
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| Authors: | LI He ZHOU Guoying LIU Junang |
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| Abstract: | Strain CSUFT0821, a pathogen causing brown rot of Agaricus bisporus, has been identified by determining the sequence of a ribosomal DNA internal transcribed spacer (ITS) and comparing with corresponding sequences deposited in GenBank. Homology was highest (99%) with an ITS sequence from Mycogone perniciosa (EU380317). A phylogenetic tree constructed on the basis of ITS sequences revealed very close proximity between EU380317 and the ITS sequence from strain CSUFT0821, and confirmed that the latter was M. perniciosa. |
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| Keywords: | Agaricus bisporus brown rot internal transcribed spacers (ITS) molecular identification |
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