Oxidative stress and antioxidant responses in juvenile Brazilian flounder <Emphasis Type="Italic">Paralichthys orbignyanus</Emphasis> exposed to sublethal levels of nitrite |
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| Authors: | Lucas Campos Maltez Luis André Luz Barbas Lilian Fiori Nitz Lucas Pellegrin Marcelo Hideo Okamoto Luís André Sampaio José Maria Monserrat Luciano Garcia |
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| Institution: | 1.Laboratório de Aquacultura Continental, Instituto de Oceanografia,Universidade Federal do Rio Grande – FURG,Rio Grande,Brazil;2.Laboratório de Aquacultura de Espécies Tropicais,Instituto Federal de Educa??o Ciência e Tecnologia do Pará – Campus Castanhal,Castanhal,Brazil;3.Laboratório de Piscicultura Estuarina e Marinha, Instituto de Oceanografia,Universidade Federal do Rio Grande – FURG,Rio Grande,Brazil;4.Laboratório de Bioquímica Funcional de Organismos Aquáticos, Instituto de Oceanografia,Universidade Federal do Rio Grande – FURG,Rio Grande,Brazil |
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| Abstract: | This study evaluated the effects of short-term exposure to sublethal levels of nitrite on oxidative stress parameters and histology of juvenile Brazilian flounder Paralichthys orbignyanus. An assessment of fish recovery was also performed. Fish were exposed to 0.08 (control), 5.72, 10.43, and 15.27 NO2-N mg L?1 for 10 days followed by the same recovery time. Gill, liver, and muscle samples were collected after 1, 5, and 10 days of exposure and after recovery for the measurement of antioxidant capacity against peroxyl radicals (ACAP), glutathione-S-transferase (GST) activity, content of non-protein (NPSH) and protein thiols (PSH), and lipid peroxidation levels by thiobarbituric acid-reactive substances (TBARS) content. Nitrite exposure induced alterations which compromised the overall antioxidant system (reduced ACAP and GST activity) and enhanced oxidative damage in lipids and proteins. Increases in GST activity and NPSH and PSH contents were also demonstrated. The recovery period allowed for resumption of basal levels for all (treatment 5.72 NO2-N mg L?1) or some of the evaluated parameters (other treatments). In conclusion, exposure to nitrite concentrations from 5.72 to 15.27 NO2-N mg L?1 induced oxidative stress and antioxidant responses in juvenile Brazilian flounder. The 10-day recovery period was sufficient for a complete resumption of basal physiological condition of fish exposed to concentrations of up to 5.72 NO2-N mg L?1. |
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