| 离子束诱变小麦高蛋白突变体的SSR分析 |
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| 引用本文： | 陈秋芳,焦浈,押辉远,秦广雍.离子束诱变小麦高蛋白突变体的SSR分析[J].核农学报,2008,22(6):745-749. |
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| 作者姓名： | 陈秋芳 焦浈 押辉远 秦广雍 |
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| 作者单位： | [1]郑州大学河南省离子束生物工程重点实验室,河南郑州450052 [2]洛阳师范学院生命科学系,河南洛阳471022 |
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| 基金项目： | 河南省重大科技攻关项目(0520010101) |
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| 摘 要： | 利用SSR标记技术,对从离子束注入小麦的后代中选到的株高变矮、蛋白含量提高的突变体M6进行分析,其中有两对引物扩增出与对照有差异的DNA片段。将差异DNA片段回收、克隆、测序、序列对比,结果显示,与对照相比,突变体扩增的DNA片段主要表现为碱基的置换和缺失,其中有一个DNA片段序列与对照同源性较低,经BLASTn搜索,发现该变异序列与普通小麦(Triticum aestivum)BAC克隆的一段序列匹配率达到95%。同时发现该序列与圆锥小麦(Triticum turgidum)高分子量麦谷蛋白(Agenome HMW glutenin)位点中的一段序列匹配率也达到95%。
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| 关 键 词： | 离子束注入 小麦 突变体 SSR |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2009-12-31 |
SSR-MARKER ANALYSIS OF THE HIGH PROTEIN CONTENT MUTANTS OF WHEAT BY ION BEAM IMPLANTATION |
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| CHEN Qiu-fang,JIAO Zhen,YA Hui-yuan,QIN Guang-yong.SSR-MARKER ANALYSIS OF THE HIGH PROTEIN CONTENT MUTANTS OF WHEAT BY ION BEAM IMPLANTATION[J].Acta Agriculturae Nucleatae Sinica,2008,22(6):745-749. |
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| Authors: | CHEN Qiu-fang JIAO Zhen YA Hui-yuan QIN Guang-yong |
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| Abstract: | Wheat was implanted by ion beam and its mutant offspring plants were obtained through field selections and protein determination in the sixth generation.The mutants were with characteristic of high-protein content and dwarf.The mutants were analyzed by using SSR marker.Deletion,substitution of SSR various amplification products occured in two SSR loci in the mutants.Comparing the sequence of mutants and CK,one mutant showed low degree of sequence homology.Using BLASTn,the sequence shared 95% homology with D... |
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| Keywords: | ion-bean implantation wheat mutant simple sequence repeat |
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