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维生素C对泥鳅幼鱼生长和免疫的影响
引用本文:武迪,管婷婷,张思雨,赵兴文.维生素C对泥鳅幼鱼生长和免疫的影响[J].水产科学,2016(6):658-662.
作者姓名:武迪  管婷婷  张思雨  赵兴文
作者单位:大连海洋大学,辽宁大连,116023
摘    要:水温21~26℃下,分别在基础饲料中添加不同含量的维生素C(以抗坏血酸多聚磷酸酯为维生素C源,添加量分别为0、50、120、240、360、500mg/kg),研究维生素C对(1.996±0.176)g泥鳅幼鱼的影响。试验结果表明,随着维生素C添加量的增加,泥鳅幼鱼的特定生长率呈现先升后降趋势,并在添加量为132.2mg/kg时达到最高值,显著高于其他几个试验组(P0.05)。组织和肌肉中的维生素C积累量随着饲料中维生素C添加量的增加呈先升后降趋势,在132.2mg/kg时和248.8mg/kg时分别达到最高。随着饲料中维生素C含量的增加,溶菌酶活性也呈现出先升后降趋势,在362.6mg/kg时达到最高,显著高于其他组(P0.05);血清总抗氧化能力呈上升趋势,以特定生长率和血清溶菌酶含量为指标,做折线模型回归分析最终求得泥鳅幼鱼饲料中维生素C的最适添加量分别为95mg/kg和423.3mg/kg。

关 键 词:泥鳅幼鱼  维生素C  肝脏和肌肉积累量  非特异性免疫

Effect of Dietary Vitamin C on Growth and Immunity of Juvenile Loach
Abstract:The basal diet were supplemented with 0 ,50 ,120 ,240 ,360 ,500 mg/kg vitamin C(L‐ascorbyl‐2‐polyphosphate) ,and fed loach (Misgurnus anguillicaudatus) with initial body weight of (1 .996 ± 0 .176) g twice (8:00 and 20 :00) a day for ten weeks in 18 grass jars (85 cm × 55 cm × 50 cm) containing 30 fish to investigate the effect of dietary vitamin C on growth performance (survival ,specific growth rate) ,immunity (total antioxidant capacity and activities of lysozyme and superoxide dismutase )and the tissue concentration in juvenile loach .The results showed that with the extension to the vitamin C accumulation ,the specific grow th rate (SGR) of juvenile loach increased obviously from 0 mg/kg to 132 .2 mg/kg ,significantly better than the other groups(P<0 .05) .With the increase in the vitamin C content ,the concentration in hepato‐pancreas and muscle was first increased ,and then fell down with the maximum value of 132 .2 mg/kg and 248 .8 mg/kg .So did the lysozyme ,with the maximum level of 362 .6 mg/kg(P<0 .05) ,and increase in to‐tal antioxidant capacity .We used the specific grow th rate and lysozyme activity to draw the Broken Line E‐quation to describe the best suitable supplement of vitamin C ,at the optimal dose of 95 mg/kg and 423 .3 mg/kg .
Keywords:Misgurnus anguillicaudatus  vitamin C  tissue concentration  nonspecific immunity
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