| 贯众、当归、黄芪对半滑舌鳎血液抗氧化指标的影响 |
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| 引用本文： | 葛婧,郭永军,王庆奎,徐大为,孙学亮,陈成勋,邢克智.贯众、当归、黄芪对半滑舌鳎血液抗氧化指标的影响[J].水产科学,2016(5):492-497. |
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| 作者姓名： | 葛婧 郭永军 王庆奎 徐大为 孙学亮 陈成勋 邢克智 |
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| 作者单位： | 天津农学院水产学院，天津市水产生态及养殖重点实验室，天津300384 |
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| 基金项目： | 国家星火计划项目(2013GA610002)；国家自然科学基金面上项目(31270456)；天津市科技支撑计划项目(13ZCZDNC00700)；天津市高等学校创新团队基金资助项目(TD12－5018)． |
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| 摘 要： | 在水温(21±1)℃和盐度20±1下,将体质量(95.45±5.29)g的半滑舌鳎饲养在78cm×58cm×46cm水槽中,投喂按0、5、10、15g/kg添加贯众、当归、黄芪的饲料,每箱20尾,每个水平3个重复,7d、14d和21d时尾柄采血,测定总超氧化物歧化酶和过氧化氢酶活性以及总抗氧化能力、丙二醛、总胆固醇和甘油三酯含量。试验结果表明,14d时,贯众剂量为15g/kg组的半滑舌鳎血液中总超氧化物岐化酶活力显著高于对照组(P0.05),14d和21d时,各剂量组丙二醛含量均低于对照组;14d时,当归剂量为5g/kg组的半滑舌鳎血液中总超氧化物岐化酶活力达到最高值;21d时,黄芪剂量为10g/kg组的丙二醛含量值显著低于对照组(P0.05),21d时,黄芪剂量为5g/kg组的半滑舌鳎血液中总超氧化物岐化酶活力达到最高值,不同剂量的黄芪对过氧化氢酶活力有显著影响,但均低于对照组(P0.05);7d时,各组总抗氧化能力达到最高值。试验表明,单独添加贯众、当归及黄芪均不同程度地影响半滑舌鳎血液的抗氧化指标。
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| 关 键 词： | 贯众 当归 黄芪 半滑舌鳎 抗氧化指标 |
Effect of Cy rtomium rhizome,Angelica sinensis,and Astragalus on Blood Antioxidant Parameters in Half-smooth Tongue-sole(Cynoglossus semilaevis) |
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| Abstract: | A feeding trial was conducted to evaluate effect of Cyrtomium rhizome ,Angelicasinensis ,and Astrag‐alus on blood parameters including activities of total superoxide dismutase (T‐SOD) ,total antioxidant capacity (T‐AOC) ,and catalase (CAT ) ,and levels of malonaldehyde (MDA) ,triglyceride (TG) ,and total cholesterol (T‐CHO) in half‐smooth tongue‐sole (Cynoglossus semilaevis) .Juvenile half‐smooth tongue‐sole with body weight of (95 .45 ± 5 .29) g were reared in tanks of each 78 cm × 58 cm × 46 cm at a rate of 20 fish/tank and fed diets contai‐ning C .rhizome ,A .sinensis ,or Astragalus at different doses of 0 g/kg diet ,5 g/kg diet ,10 g/kg diet ,and 15 g/kg diet for 21 days with triplication at water temperature of (21 ± 1) ℃ and a salinity of 20 ± 1 .The activities of T‐SOD ,T‐AOC ,and CAT ,and levels of MDA ,TG ,and T‐CHO were detected in blood of the half‐smooth tongue‐sole in 7 days ,14 days and 21 days during the rearing period .It was found that the fish fed the diet supple‐mented with C .rhizome at a dose of 15 g/kg diet had significantly higher T‐SOD activity than the fish fed the con‐trol diet in 14 days ,and had significantly lower MDA content than the fish fed the control group in 14 and 21 days (P<0 .05) .The maximal T‐SOD activity was observed in the fish fed the diet supplemented with A .sinensis at a dose of 5 g/kg diet in 14 days .There were significantly lower MDA content in the fish fed the diet supplemented with A .sinensis at a rate of 10 g/kg diet than in the fish in the control group in 21 days (P<0 .05) .Diet supple‐mented with A stragalus at a dose of 5 g/kg diet resulted in significantly higher T‐SOD activity and significantly lower CAT activity in 21 days compared to the control diet (P<0 .05) .There was significantly higher T‐AOC ac‐tivity in the fish fed the diet containing different doses of Astragalus in 7 days .The findings indicated that C .rhi‐zome ,A .sinensis ,and Astragalus supplementation affected to some extent on blood antioxidant parameters in half‐smooth tongue‐sole . |
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| Keywords: | Cyrtomium rhizome Angelicasinensis Astragalus Cynoglossus semilaevis antioxidant pa-rameter |
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