| 基于COⅠ基因分析辽东湾海蜇群体遗传多样性 |
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| 引用本文： | 李玉龙,王彬,王文波,董婧.基于COⅠ基因分析辽东湾海蜇群体遗传多样性[J].水产科学,2016(4):404-409. |
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| 作者姓名： | 李玉龙 王彬 王文波 董婧 |
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| 作者单位： | 辽宁省海洋水产科学研究院，辽宁省海洋生物资源与生态学重点实验室，辽宁大连116023 |
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| 基金项目： | 国家自然科学基金资助项目(31400406)；国家海洋公益性行业科研专项项目(201405010)． |
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| 摘 要： | 为研究海蜇群体的遗传多样性状况,采用PCR扩增获得20个海蜇野生群体样品的线粒体COⅠ基因部分序列,并结合GenBank上其他15个海蜇样品的同源序列,对其序列变异和遗传分化进行分析。结果显示,35条序列的A、T、C、G碱基含量分别为26.7%、36.4%、18.8%、18.1%;在长度624bp的线粒体COⅠ基因片段中共发现21个多态位点,定义了17种单倍型,单倍型多态性为0.91±0.03,核苷酸多态性为0.0056±0.0032。同其他几种大型水母相比,海蜇种群的遗传多样性处于较高或中等水平。研究结果表明,不同海蜇种群尤其是相距较远的群体在其分布范围内可能存在遗传分化现象。
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| 关 键 词： | 海蜇 线粒体DNA COⅠ基因 遗传多样性 |
Genetic Diversity Analysis of Jellyfish Rhopilema esculentum in Liaodong Bay Based on the COⅠ Sequence |
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| Abstract: | The 624 bp fragments of mitochondrial cytochrome oxidase Ⅰ(COⅠ) gene were sequenced and analyzed in 20 individuals jellyfish Rhopilema esculentum collected from Liaodong Bay to investigate the genetic variation and diversity .In addition ,the homologous sequences were analyzed in other 15 individu‐als sampled from the Bohai and Yellow Seas and Sea of Japan .The average contents were found to be 26 .7% in A ,36 .4% in T ,18 .8% in C and 18 .1% in G for COⅠ sequence .A total of 21 polymorphic nu‐cleotide sites were detected ,which defined 17 haplotypes .Compared with several other giant jellyfish spe‐cies ,the variation level of the jellyfish was moderate to high with H of 0 .91 ± 0 .03 and π of 0 .0056 ± 0 .0032 .With the aid of the homologous sequences retrieved from GenBank ,both phylogenetic and molec‐ular variance analysis supported significant differentiation among jellyfish populations in Sea of Japan and the Bohai Sea and Yellow Sea populations . |
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| Keywords: | Rhopilema esculentum mtDNA COⅠ gene genetic diversity |
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