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大竹蛏幼贝滤水率的响应面法分析
引用本文:姜北,董颖,高杉,王摆,曹琛,张乾,郝咏芳,王永波,周遵春.大竹蛏幼贝滤水率的响应面法分析[J].水产科学,2016(4):313-320.
作者姓名:姜北  董颖  高杉  王摆  曹琛  张乾  郝咏芳  王永波  周遵春
作者单位:1. 辽宁省海洋水产科学研究院,辽宁大连,116023;2. 营口市水产科学研究所,辽宁营口,115003;3. 营口现代渔业科技产业园,辽宁营口,115001
基金项目:辽宁省海洋与渔业厅项目(201603,201301);辽宁省科技计划项目(2015103044).
摘    要:采用三因素三水平中心组合试验设计方案,研究光照度、盐度、pH及三者交互作用对大竹蛏幼贝滤水率的影响,并进行响应面分析,探寻大竹蛏幼贝最适生长条件组合,构造大竹蛏幼贝滤水率模型。试验期为30d,试验光照梯度为2000lx、1000lx、0,盐度梯度为30、25、20,pH梯度为9.00、8.00、7.00。结果显示,光照度、盐度及pH三者对大竹蛏幼贝滤水率有显著交互作用(P0.05)。通过Design-Expert 8.0软件对数据进行二次多元回归,拟合得到大竹蛏幼贝滤水率y对编码自变量A(光照度)、B(盐度)和C(pH)的二次多元回归方程:y=1.62+0.084A+0.04B-0.14C-0.38A2-0.65B2-0.68C2(r2=0.9821),软件模拟最适大竹蛏幼贝生长的条件组合为光照度1109.17lx,盐度25.12,pH7.89。

关 键 词:大竹蛏  光照度  盐度  pH  滤水率  响应面分析

Optimization of Filtration Rate in Juvenile Razor Clam Solen grandis by Response Surface Methodology
Abstract:The joint effects of illumination ,salinity and pH on filtration rate of juvenile razor clam Solen grandis were studied using Box‐Behnken experimental design and response surface methodology ( RSM ) under laboratory conditions ,aiming to quantifying the relationship between the filtration rate and the a‐bove three factors ,and to the optimal factor combination through statistical optimization approach .The re‐sults showed that there were significant interaction effects between illumination and salinity ,and between illumination and pH on the filtration rate of razor clam juveniles (P<0 .05) .The multivariate non‐linear model is described as y =1 .62+0 .084A+0 .04B -0 .14C -0 .38A2 -0 .65B2 -0 .68C2 (r2 =0 .9821) ,the optimal conditions for filtration were found under conditions of illumination of 1109 .17 lx ,salinity of 25 .12 and pH=7 .89 .T he findings indicate that the proposed model is reasonably accurate and can be used for describing and predicting the grow th features of juvenile razor clam in different environmental factors .
Keywords:Solen grandis  illumination  salinity  pH  filtration rate  response surface methodology
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