| 玉米禾谷镰孢穗腐病抗性鉴定接种方法比较 |
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| 引用本文： | 贾娇,张伟,孟玲敏,白雪,吴宏斌,王义生,张玉榕,苏前富.玉米禾谷镰孢穗腐病抗性鉴定接种方法比较[J].玉米科学,2022,30(5):149-155. |
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| 作者姓名： | 贾娇 张伟 孟玲敏 白雪 吴宏斌 王义生 张玉榕 苏前富 |
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| 作者单位： | 吉林省农业科学院/农业部东北作物有害生物综合治理重点实验室, 吉林 公主岭 136100 |
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| 基金项目： | 吉林省科技发展计划项目(20210202121NC)、国家玉米产业技术体系项目(CARS-02) |
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| 摘 要： | 通过子粒注射法和花丝通道法，在玉米吐丝初期至乳熟后期间隔 5～127 d接种 3个不同玉米品种，明确东北地区玉米禾谷镰孢穗腐病抗性鉴定的最佳接种方法。结果发现，禾谷镰孢在玉米吐丝期至乳熟期均能侵染玉米子粒和花丝。子粒注射法在玉米吐丝 1～12 d内接种的病情指数显著高于 16 d以后接种的处理；花丝通道法在玉米吐丝 1～5 d内的病情指数显著高于 12 d以后接种的处理。子粒注射法的抗性鉴定结果为吐丝 1 d接种的果穗表现为感病，吐丝 5～12 d为中抗或抗病，吐丝 16 d之后为抗病或高抗；花丝通道法接种的结果为吐丝 1 d为中抗或抗，吐丝 5 d及以后为抗或高抗。结果表明，子粒注射法在玉米吐丝 10～15 d左右接种发病稳定、抗感差异明显、鉴定结果准确。
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| 关 键 词： | 玉米 穗腐病 禾谷镰孢 接种方法 抗性评价 |
| 收稿时间： | 2021/7/20 0:00:00 |
Comparison of Two Inoculation Methods for Resistance of Fusarium Graminearum Ear Rot |
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| JIA Jiao,ZHANG Wei,MENG Ling-min,BAI Xue,WU Hong-bin,WANG Yi-sheng,ZHANG Yu-rong,SU Qian-fu.Comparison of Two Inoculation Methods for Resistance of Fusarium Graminearum Ear Rot[J].Journal of Maize Sciences,2022,30(5):149-155. |
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| Authors: | JIA Jiao ZHANG Wei MENG Ling-min BAI Xue WU Hong-bin WANG Yi-sheng ZHANG Yu-rong SU Qian-fu |
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| Institution: | Jilin Academy of Agricultural Sciences/Key Laboratory of Integrated Pest Management on Crops in Northeast, Ministry of Agriculture, Gongzhuling 136100, China |
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| Abstract: | Three different maize varieties were inoculated at an interval of 5-12 days from the early stage of maize silking to the late stage of milk maturity through the kernel injection method and the silk channel method, and the best inoculation method for the identification of Fusarium ear rot resistance of maize grains in Northeast China was determined. The results showed that Fusarium graminearum can infect maize kernels and silks from the maize silking stage to milk maturity stage, the disease index of maize kernels inoculated within 1-12 days of maize silking through the kernel injection method is significantly higher than that of the treatments inoculated after 16 days. The disease index of maize kernels inoculated within 1-5 days of maize silking by the silk channel method is significantly higher than that of the treatment after 12 days. The resistance identification result of the kernel injection method is that the ears inoculated on the first day of silking are susceptible, the ears inoculated after 5-12 days of silking are moderately resistant or resistant, and after 16 days of silking, they are resistant or high resistant. The result of inoculation by the silk channel method is that they are moderately resistant or resistant after silking for 1 day, silking for 5 days and later is resistant or high resistant. The results showed that the maize ears that were inoculated at 10- 15 days after maize silking by the grain injection method had stable disease, obvious difference in resistance and susceptibility, and accurate identification results. |
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| Keywords: | Maize Ear rot Fusarium graminearum Inoculation method Resistance evaluation |
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