Identification and Purity Test of Super Hybrid Rice with SSR Molecular Markers |
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| Authors: | XIN Ye-yun ZHANG Zhan XIONG Yi-ping YUAN Long-ping |
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| Institution: | ;1.School of Life Science and Technology, Central South University, Changsha 410083, China; ;2.China National Hybrid Rice Research and Developmental Center, Changsha 410125, China |
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| Abstract: | Five super hybrid rice combinations, i.e. HYS-1/R105, Pei'ai 64S/E32, Liangyoupeijiu (Pei'ai 64S/9311), 88S/0293, and J23A/Q611, and their parental lines were tested by means of SSR analysis. A total of 144 SSR primer pairs distributed on 12 rice chromosomes were used, out of which 47 detected polymorphism among the tested rice lines. Among all these primers, RM337 and RM154 produced polymorphic patterns in four or more of the tested experimental materials respectively, and they could distinguish among most rice genotypes tested. Twenty-four primer pairs, two on each rice chromosome, were selected to make a reference SSR marker-based fingerprinting for the rice lines. For most of the primer pairs, F1 hybrids mainly showed complementary pattern of both parents, which could be very useful to distinguish the F1 from its parental lines. In addition, 5 primer pairs were selected as special primer pairs for five hybrid rice combinations respectively. By combining the rapid, simple method on DNA extraction, it is suggested that SSR technique has wide prospective in variety authentication and purity identification. |
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| Keywords: | SSR molecular marker super hybrid rice identification purity |
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