| 微卫星标记BM1329在湘东黑山羊中的遗传多态性研究 |
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| 作者单位： | 湖南农业大学动物科学技术学院 湖南长沙410128(黄生强，袁峥嵘)，湖南生物机电职业技术学院 湖南长沙410127(欧阳叙向，刘鹤翔) |
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| 摘 要： | 利用经筛选的绵羊微卫星引物BM1329,采用聚丙烯酰胺凝胶垂直板电泳,对湘东黑山羊的BM1329微卫星基因座进行了多态性检测。结果表明:该基因座有8个等位基因,其片段大小在175bp-215bp之间,计算了该基因座各等位基因频率、基因型频率及其平均基因杂合度(0.8305)、多态信息含量(0.8090)和有效等位基因数(5.9001)。说明在湘东黑山羊中微卫星标记BM1329基因座具有丰富的遗传多态性,可用于山羊遗传多态性的评估,也为山羊的选育、保存和利用提供了一定的科学理论依据。
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| 关 键 词： | 微卫星标记 遗传多态性 山羊 |
Analysis of DNA Polymorphisms by Microsatellite BM1329 in the Black Goats in East Hunan Province |
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| Authors: | HUANG Sheng-qiang YUAN Zheng-rong College of Animal Science and Technology Hunan Agricultural University Changsha China OU YANG Xu-xiang LIU He-xiang Hunan Biological and Electromechanical Polytechnic Changsha China |
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| Institution: | HUANG Sheng-qiang YUAN Zheng-rong College of Animal Science and Technology,Hunan Agricultural University,Changsha 410128,China OU YANG Xu-xiang LIU He-xiang Hunan Biological and Electromechanical Polytechnic,Changsha 410127,China |
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| Abstract: | By using the selected microsatellite BM1329 of the sheep,the polymorphisms at BM1329 lo- cus was analyzed in the black goats in the east of Hunan Province.The results show that the size of the al- leles of the locus was between 175bp and 215bp and the number of the allele was 8.Then the allele fre- quencies,the type of allele,heterozygosity(0.8305),polymorphisms information content(PIC 0.8090) and effective number of alleles(5.9001)of the black goats in the east of Hunan Province were calculated and analyzed.The result indicates that the genetic polymorphisms by using microsatellite BM1329 in the black goats in the east of Hunan Province can evaluate the genetic polymorphism of species of goats,and it provided the precious theoretic basis for the selection,conservation and utilization of goats breeds. |
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| Keywords: | microsatellite marker genetic polymorphisms goat |
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