| 绒山羊瘤胃甲烷菌mcrA基因的RFLP分析(英文) |
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| 引用本文： | 李长青,金海,郭雪峰,李华伟,栗原光规.绒山羊瘤胃甲烷菌mcrA基因的RFLP分析(英文)[J].农业科学与技术,2008,9(3):49-52. |
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| 作者姓名： | 李长青 金海 郭雪峰 李华伟 栗原光规 |
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| 作者单位： | 内蒙古农牧业科学院生物中心;内蒙古农业大学动物科学与医学学院;日本国独立行政法人农业·食品产业技术研究机构畜产草地研究所 |
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| 摘 要： | Objective] The aim of this study is to understand the population composition of methanogens in rumen fluid of grazing Inner Mongolian cashmere goat. Method] Total DNAs of various bacteria in rumen fluid were isolated for PCR amplification using the specifically designed primers based on conservative mcrA sequence of methanogens; then mcrA specific clone library was accordingly established. The restriction fragment length polymorphism(RFLP) of the library was further analyzed by digestion of restriction enzyme Taq I. Result] One hundred and five randomly selected specific colonies were classified into six RFLP types, among which the dominant type accounts for 38%, and other types account for 27%, 18%, 5.5% and 4.5%, respectively. Conclusion] There are at least six different methanogens in rumen fluid of grazing Inner Mongolian cashmere goat.
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| 关 键 词： | Casmere goat mcrA clone library Rumen fluid RFLP |
RFLP Analysis of Methanogens mcrA Gene in Rumen of Cashmere Goat |
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| LI Chang-qing,JIN Hai,GUO Xue-feng,LI Hua-wei,Mitsunori KURIHARA.RFLP Analysis of Methanogens mcrA Gene in Rumen of Cashmere Goat[J].Agricultural Science ＆ Technology,2008,9(3):49-52. |
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| Authors: | LI Chang-qing JIN Hai GUO Xue-feng LI Hua-wei Mitsunori KURIHARA |
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| Institution: | LI Chang-qing1, JIN Hai1, GUO Xue-feng2, LI Hua-wei2, Mitsunori KURIHARA3 1.Biotechnology Research Center, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031; 2. College of Animal Science and Medical Science, Inner Mongolia Agricultural University, Hohhot 010018; 3. Division of Animal Sciences, National Institute of Agrobiological Sciences,Ibaraki, Japan 305-0901 |
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| Abstract: | Objective] The aim of this study is to understand the population composition of methanogens in rumen fluid of grazing Inner Mongolian cashmere goat. Method] Total DNAs of various bacteria in rumen fluid were isolated for PCR amplification using the specifically designed primers based on conservative mcrA sequence of methanogens; then mcrA specific clone library was accordingly established. The restriction fragment length polymorphism(RFLP) of the library was further analyzed by digestion of restriction enzyme Taq I. Result] One hundred and five randomly selected specific colonies were classified into six RFLP types, among which the dominant type accounts for 38%, and other types account for 27%, 18%, 5.5% and 4.5%, respectively. Conclusion] There are at least six different methanogens in rumen fluid of grazing Inner Mongolian cashmere goat. |
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| Keywords: | Casmere goat mcrA clone library Rumen fluid RFLP |
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