| 外源一氧化氮对低温胁迫下苦瓜幼苗生长及部分抗逆指标的影响 |
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| 引用本文： | 杜卓涛,杨衍,朱国鹏,田丽波,商桑.外源一氧化氮对低温胁迫下苦瓜幼苗生长及部分抗逆指标的影响[J].浙江农业学报,2016,28(5):776. |
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| 作者姓名： | 杜卓涛 杨衍 朱国鹏 田丽波 商桑 |
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| 作者单位： | （1.海南大学 园艺园林学院/热带作物种质资源保护与开发利用教育部重点实验室，海南 海口 570228； 2.中国热带农业科学院 热带作物品种资源研究所，海南 儋州 571737） |
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| 摘 要： | 为探讨外源一氧化氮（NO）提高苦瓜抗冷性的内在机制，以碧绿苦瓜幼苗为试材，对其施加不同浓度（0，01，05，10，15和20 mmol·L-1）的外源NO供体（SNP）并进行低温（8 ℃）处理，研究外源NO对低温胁迫下苦瓜幼苗的生长、相对电导率、丙二醛（MDA）、脯氨酸含量以及过氧化物酶（POD）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性的影响。结果显示：SNP处理提高了茎粗/株高和叶面积/根体积的比值，降低了苦瓜幼苗叶片相对电导率和 MDA含量，提高了叶片脯氨酸含量和SOD，CAT，POD三种酶的活性，从而降低低温胁迫对苦瓜幼苗的伤害。低浓度的SNP处理的苦瓜幼苗抗冷效果好于高浓度的，尤其以05 mmol·L-1 SNP处理效果最佳。结果表明：低温胁迫下，适宜浓度的外源NO通过提高抗氧化酶活性，促进渗透调节物质的合成，降低膜透性和膜脂过氧化水平，保护了细胞膜结构的稳定性，从而提高苦瓜幼苗抗低温胁迫的能力，促进了苦瓜幼苗的生长。
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| 关 键 词： | 苦瓜 外源NO（SNP） 低温胁迫 防御酶 |
Effects of exogenous NO on plant growth and resistant characteristics of bitter melon seedlings under low temperature stress |
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| DU Zhuo tao,YANG Yan,ZHU Guo peng,TIAN Li bo,SHANG Sang.Effects of exogenous NO on plant growth and resistant characteristics of bitter melon seedlings under low temperature stress[J].Acta Agriculturae Zhejiangensis,2016,28(5):776. |
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| Authors: | DU Zhuo tao YANG Yan ZHU Guo peng TIAN Li bo SHANG Sang |
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| Institution: | （1. College of Horticulture and Landscape/Key Laboratory of Protection and Developmental Utilization of Tropical Crop Germplasm Resources， Hainan University， Haikou 570228， China; 2. Tropical Pasture Research Center， Chinese Acdemy of Tropical Agricultural Sciences, Danzhou 571737， China） |
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| Abstract: | For exploring the internal mechanism of exogenous NO in improving the cold resistance of bitter melon， in this experiment， the variety of Bilv was taken as test material， different concentrations of sodium nitroprusside （SNP） （0， 01， 05， 10， 15 and 20 mmol·L-1） was employed as exogenous nitric oxide （NO） donor to study the effects of different concentrations of exogenous nitric oxide （NO） on the plant growth， relative electrical conductivity， MDA content， proline content and the activities of POD， SOD， CAT of bitter melon seedlings under 8 ℃ low temperature stress. The results showed that the treatment of SNP could improve plant height/stem diameter and leaf area/root volume; reduce the relative electrical conductivity and content of MDA; increase proline content and the activities of POD， SOD and CAT of the bitter melon seedlings under low temperature stress to reduce the damage caused by low temperature. It was also found that the cold resistance effect of treatment with low concentration of SNP was better than that with high concentration， especially the treatment with 05 mmol·L-1 SNP was the best. It indicated that the exogenous NO with proper concentration could promote the plant growth and enhance the bitter melon seedlings adaptability for low temperature stress through improving the activities of defensive enzymes， increasing the osmotic adjustment substance contents， reducing membrane lipid peroxidation and protecting the stability of cell membrane structure. |
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| Keywords: | bitter melon exogenous NO （SNP） low temperature stress defensive enzyme |
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