| 免疫增强剂对猪伪狂犬灭活疫苗的免疫增强作用 |
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| 引用本文： | 耿文学,唐波,华涛,侯继波,张道华,许家荣.免疫增强剂对猪伪狂犬灭活疫苗的免疫增强作用[J].浙江农业学报,2016,28(11):1828. |
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| 作者姓名： | 耿文学 唐波 华涛 侯继波 张道华 许家荣 |
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| 作者单位： | 1.南京农业大学 动物医学院,江苏 南京 210095; 2.江苏省农业科学院 国家兽用生物制品工程技术研究中心,江苏 南京 210014 |
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| 基金项目： | 江苏省农业科技自主创新基金项目[CX(11)2047]; 农业公益性行业科研专项(201303046); 江苏省优质学科(20150101) |
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| 摘 要： | 为了评价免疫增强剂VA5对猪伪狂犬病毒灭活疫苗的免疫增强作用,将VA5与灭活疫苗混合并制成疫苗。通过常规Bartha-K61株灭活疫苗、含免疫增强剂灭活疫苗和Bartha-K61活疫苗3组免疫效力对比试验,首次免疫后14和35 d对猪进行采血,并且进行中和实验以及对相关细胞因子进行检测。结果表明,该免疫增强剂能够显著地提高猪伪狂犬灭活疫苗血清中抗体产生,同时能显著提高猪外周血淋巴细胞(PBMC)中IL-2和IFN-γ mRNA表达。交叉中和试验表明,VA5能够显著地提高猪伪狂犬灭活疫苗对伪狂犬流行变异株的中和作用。试验表明,VA5能够显著提升猪伪狂犬病毒灭活疫苗体液与细胞免疫,为研制免疫效果更好的灭活疫苗提供了依据。
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| 关 键 词： | 免疫增强剂 伪狂犬病毒灭活疫苗 Bartha-K61株 免疫效力 |
| 收稿时间： | 2016-04-18 |
Improved immune efficiency of inactivated vaccine of pseudorabies virus by immunopotentiator |
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| GENG Wen-xue,TANG Bo,HUA Tao,HOU Ji-bo,ZHANG Dao-hua,XU Jia-rong.Improved immune efficiency of inactivated vaccine of pseudorabies virus by immunopotentiator[J].Acta Agriculturae Zhejiangensis,2016,28(11):1828. |
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| Authors: | GENG Wen-xue TANG Bo HUA Tao HOU Ji-bo ZHANG Dao-hua XU Jia-rong |
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| Institution: | 1. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China;
2. National Research Center of Veterinary Biologicals Engineering and Technology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China |
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| Abstract: | To evaluate the effect of immunopotentiator VA5 on immunity potency of inactive vaccine of pseudorabies virus (PRV), inactive vaccine was mixed with VA5. The comparison test of the immunity potency was conducted by using conventional inactive vaccine of Bartha-K61 strain of PRV, inactive vaccine mixed with VA5 and attenuated vaccine of Bartha-K61 strain. Blood samples were collected at 14 and 35 days after the first immunization, respectively. Neutralization experiments and cytokine detection were performed. The results showed that, compared with live and inactive vaccine of Bartha-K61 strain of PRV, the inactive vaccine with VA5 could induce higher neutralizing antibody against the heterologous strain of PRV and increase the expression levels of IL-2 and IFN-γ mRNA in porcine peripheral blood lymphocytes (PBMC). Neutralization test showed that VA5 could significantly increase the level of neutralizing antibody against the epidemic variation strain of PRV. This experiment showed that VA5 could improve the humoral and cellular immunity of inactive PRV vaccine, and provide a basis for paving the better immune effect of inactivated PRV vaccine. |
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| Keywords: | immunopotentiator inactive PRV vaccine Bartha-K61 strain immunity potency |
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