| α菠菜甾醇对小鼠小肠段药物转运体P-gp和PEPT1表达的影响 |
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| 引用本文： | 吴丽娜,赵玲,叶刚,李英伦.α菠菜甾醇对小鼠小肠段药物转运体P-gp和PEPT1表达的影响[J].浙江农业学报,2016,28(6):944. |
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| 作者姓名： | 吴丽娜 赵玲 叶刚 李英伦 |
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| 作者单位： | （四川农业大学 动物医学院，四川 成都610000） |
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| 摘 要： | 以酒石酸泰乐菌素为底药，探讨α 菠菜甾醇对小鼠小肠段药物转运体P gp和PEPT1表达的影响。48只小鼠分为4组：α 菠菜甾醇组，酒石酸泰乐菌素组，α 菠菜甾醇和酒石酸泰乐菌素混合组，生理盐水对照组。每组按0.2 mL·10 g-1体质量的剂量每天灌胃一次，共灌胃5 d，分别在1，3，5 d后处死1批采样。采用免疫组化的方法测定1，3，5 d的小鼠肠道上P gp和PEPT1的表达。试验结果表明，α 菠菜甾醇组和混合组相对于对照组外排性药物转运体P gp表达减少，而摄取性药物转运体PEPT1表达增多；酒石酸泰乐菌素组的外排性药物转运体P gp表达增多，摄取性药物转运体PEPT1表达减少；且都随着用药时间的延长差异增大。表明α 菠菜甾醇具有抑制药物转运体P gp表达和促进药物转运体PEPT1表达的作用。
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| 关 键 词： | &alpha 菠菜甾醇 小鼠 药物转运体 P gp PEPT1 |
Influence of α spinasterol on expression of P gp and PEPT1 in small intestine of mice |
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| WU Li na,ZHAO Ling,YE Gang,LI Ying lun.Influence of α spinasterol on expression of P gp and PEPT1 in small intestine of mice[J].Acta Agriculturae Zhejiangensis,2016,28(6):944. |
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| Authors: | WU Li na ZHAO Ling YE Gang LI Ying lun |
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| Institution: | （College of Veterinary Medicine， Sichuan Agricultural University， Chengdu 610000, China） |
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| Abstract: | In order to research the effect of α spinasterol on the intestine transporters P gp and PEPT1 when the tylenol tartrate was introduced as a substrate， 48 mice were equally divided into 4 treatment groups: α spinasterol group， tylenol tartrate group， mixed （α spinasterol and tylenol tartrate） group， and normal saline group which was used as control group. The formulations were administered by oral gavage with a dose of 0.2 mL·10 g-1 depending on body weight of mice. The small intestine samples of the dissected mice were excised at predetermined time intervals （1， 3， 5）. The expressions of P gp and PEPT1 on the mice intestines were detected by immunohistochemical method at 1， 3, 5 d, respectively. The results showed that the protein expression of P gp was distinctly reduced in α spinasterol and mixed groups， and the protein expression of PEPT1 was increased in these 2 groups compared with normal saline group. However， the protein expression of P gp was increased and the protein expression of PEPT1 was decreased in tylenol tartrate group compared with normal saline group. The difference was more obvious with time pass by. In summary， α spinasterol had an obvious impact on intestine transporters， including inhibiting P gp expression and inducing PEPT1 expression. |
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| Keywords: | α spinasterol mice drug transporter P gp PEPT1 |
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