Biological pretreatment of sugar cane bagasse for the production of cellulases and xylanases by Penicillium echinulatum |
| |
| Authors: | Marli Camassola Aldo JP Dillon |
| |
| Institution: | 1. Green Chemistry Centre of Excellence, Chemistry Department, University of York, Heslington, York, YO10 5DD, United Kingdom;2. CNAP, Department of Biology, University of York, York, Heslington, YO10 5DD, United Kingdom;3. Institute of Chemistry, University of Campinas, UNICAMP, P. O. Box 6154, 13084-971, Campinas, São Paulo, Brazil;4. Instituto de Física de São Carlos, Universidade de São Paulo/USP, Av. Trabalhador são-carlense, 400 Pq. Arnold Schimidt, 13566-590, São Carlos, São Paulo, Brazil;1. Biosystems Engineering Program, Department of Environmental Engineering and Earth Sciences, Clemson University, SC, 29634, USA;2. Department of Biological Sciences, Clemson University, SC, 29634, USA;1. Department of Chemistry at Federal University of Viçosa, Viçosa, MG 36570-000, Brazil;2. Department of Forestry Biomaterials, North Carolina State University, Campus Box 8005, Raleigh, NC 27695-8005, USA;3. Department of Forestry Engineering at Federal University of Viçosa, Viçosa, MG 36570-000, Brazil;1. Faculty Biosciences and Biomedical Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia;2. ENVIBIO Research Group, Sustainability Research Alliance, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia;3. Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Sedang, Selangor, Malaysia |
| |
| Abstract: | In this study, sugar cane bagasse was pretreated with the white rot fungus Pleurotus sajor-caju PS 2001, and this biomass was subsequently used in the production of cellulases and xylanases by the fungus Penicillium echinulatum. Despite the environmental advantages offered by this type of pretreatment, the enzymatic activity obtained with biologically pretreated sugar cane bagasse (PSCB) was lower than that of the control treatments, which were carried out with untreated sugar cane bagasse (SCB) and cellulose. For medium supplemented with PSCB, the average peak activities obtained were 0.13, 1.0, 0.18, and 0.33 U ml?1 for FPA, endoglucanase, β-glucosidases, and xylanases, respectively. For the cellulose, control values of 0.52, 1.20, 0.20, and 1.46 U ml?1, and SCB values of 0.95, 1.60, 0.21, and 1.49 U ml?1 were obtained, respectively. Although the enzymatic activities of the culture with biologically pretreated sugar cane bagasse were lower than the cultures carried out with untreated sugar cane bagasse, it should be noted that production of enzymes of the cellulase and hemicellulase complex after production of the mushrooms is another way to add value to this agricultural residue. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
