| 利用基因芯片鉴定梨品种自交不亲和基因型 |
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| 引用本文： | 江南,谭晓风,张琳,邓靖.利用基因芯片鉴定梨品种自交不亲和基因型[J].园艺学报,2014,41(10):1983. |
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| 作者姓名： | 江南 谭晓风 张琳 邓靖 |
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| 作者单位： | 1湖南工业大学包装与材料工程学院，湖南株洲 412007；2中南林业科技大学经济林培育与保护教育部重点实验室，经济林育种与栽培国家林业局重点实验室，长沙 410004 |
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| 基金项目： | 国家自然科学基金面上项目（31272124）；湖南省自然科学基金项目（12JJ6017） |
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| 摘 要： | 根据东方梨中已鉴定的46个S基因序列和S基因的结构特点，设计了86条寡核苷酸探针并制备成S基因寡核苷酸检测芯片，采用Cy3荧光修饰引物标记被检测品种的PCR产物并与芯片杂交，以检测不同品种的S基因型。结果表明：利用芯片与华梨2号、秀玉和德胜香等已知S基因型的品种杂交，杂交信号显示与各品种已知基因型相符合。利用芯片鉴定了丽江黄酸梨等27个未知S基因型的梨品种，获得了各品种的S基因型，随机选取部分品种进行DNA测序和序列分析，结果与芯片杂交结果完全一致，证明利用S基因寡核苷酸芯片鉴定梨品种S基因型结果准确可靠。
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| 关 键 词： | 梨 自交不亲和 S基因型 寡核苷酸 芯片 |
| 收稿时间： | 2014-06-26 |
Identifying Pear Cultivars S-genotypes Using Gene Chips |
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| Institution: | 1.School of Packing and Material Engineering，Hunan University of Technology，Zhuzhou，Hunan 412007，China；2Key Laboratory of Cultivation and Protection for Non-wood Forest of Ministry of Education，the Key Laboratory of Non-wood Forest Product of Forestry Ministry，Central-south University of Forestry and Technology，Changsha 410004，China |
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| Abstract: | Based on the structure characteristic of self-incompatibility gene and the gene group sequences of 46 S genes in Oriental pear cultivars，86 probes for S-RNase detections were designed and oligonucleotide genechip was established. In order to detect the S-genotype of pear cultivars，the PCR products of S-RNase were amplified by Cy3-labeled primers and hybridized with the microarray. The cultivars with known S-genotype such as Huali 2 were hybridized with the genechip and 27 cultivars with unknown S-genotype were S-genotyped using the genechip. The DNA sequenced results of Huali 2，Xiuyu and Deshengxiang pears and randomly selected unknown S-genotype cultivars were consistent with the microarray analyses. In conclusion，the oligonucleotide genechip provided an accurate and reliable method for detecting S-genotype of pear cultivars. |
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| Keywords: | pear self-incompatibility S-genotype oligonucleotide genechip |
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