| 兰属植物目标起始密码子（SCoT）遗传多样性分析 |
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| 引用本文： | 高 岭,冯尚国,何仁锋,赵妙玉,王慧中.兰属植物目标起始密码子（SCoT）遗传多样性分析[J].园艺学报,2013,40(10):2026-2032. |
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| 作者姓名： | 高 岭 冯尚国 何仁锋 赵妙玉 王慧中 |
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| 作者单位： | （杭州师范大学生命与环境科学学院，浙江省药用植物种质改良和质量监控重点实验室，杭州 310036） |
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| 摘 要： | 应用SCoT分子标记技术对兰属14个种的24个样品进行遗传多样性分析，27条引物共扩增出259条DNA条带，其中多态性条带224条，多态性百分比达86.49%，条带大小为100 ~ 2 000 bp，多数条带分布在500 ~ 1 300 bp之间。根据SCoT标记计算的遗传距离系数得到的聚类结果可知，24种兰属植物可以分为5类：A类：春兰、建兰、蕙兰、莲瓣兰；B类：墨兰、寒兰；C类：春剑；D类：兔耳兰；E类：多花兰、文山红柱兰、虎头兰、象牙白、碧玉兰、黄蝉兰，这与传统兰属分类有所区别。
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| 关 键 词： | 兰属植物 分子标记 SCoT 遗传多样性 |
| 收稿时间： | 2013-06-09 |
Application of SCoT Molecular Marker in Genetic Diversity of Cymbidium |
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| GAO Ling,FENG Shang-guo,HE Ren-feng,ZHAO Miao-yu,WANG Hui-zhong.Application of SCoT Molecular Marker in Genetic Diversity of Cymbidium[J].Acta Horticulturae Sinica,2013,40(10):2026-2032. |
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| Authors: | GAO Ling FENG Shang-guo HE Ren-feng ZHAO Miao-yu WANG Hui-zhong |
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| Institution: | （College of Life and Environmental Sciences，Hangzhou Normal University，Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants，Hangzhou 310036，China） |
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| Abstract: | In this research，SCoT markers were applied to detect the genetic diversity among 24 Cymbidum samples of 14 species. Two hundred and fifty-nine DNA bands were generated by 27 primers including 224 polymorphic bands. The polymorphic percent is 86.49%. Strip size was from 100 bp to 2 000 bp，and most of the bands were concentrated between 500 bp and 1 300 bp. Dendrograms generated from coefficient of genetic distance between species on the basis of SCoT markers showed that the germplasms were divided into 5 clusters. Cluster A was composed of C. georingii，C. ensifolium，C. faberi，and C. tortosepalum；Cluster B included C. sinese and C. kanran；C. goeringii var. ongibracteatum was cluster C；C. lancifolium belonged to cluster D；Cluster E was composed of C. floribundum，C. wenshanense，C. hookerianum，C. maguanense，C. lowianum and C. iridioides. The research showed that part of the result was different from the traditional classification of Cymbidium. |
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| Keywords: | Cymbidum molecular marker Cymbidum molecular marker SCoT genetic diversity |
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