| 利用沙冬青抗寒基因AmEBP1 转化杏的研究 |
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| 引用本文： | 牛庆霖,曹帮华,王玉山,刘 静,赵进红,罗 磊,黄艳艳,冯殿齐.利用沙冬青抗寒基因AmEBP1 转化杏的研究[J].园艺学报,2014,41(5):825-831. |
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| 作者姓名： | 牛庆霖 曹帮华 王玉山 刘 静 赵进红 罗 磊 黄艳艳 冯殿齐 |
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| 作者单位： | 1 山东农业大学农业生态与环境重点实验室，山东泰安 271018；2 泰安市泰山林业科学研究院，山东泰安 271000 |
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| 基金项目： | 国家林业局‘948’项目（2008-4-20） |
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| 摘 要： | 利用花粉管导入法，将沙冬青抗寒基因AmEBP1 转化到‘大果’杏幼胚中，在改良WPM 培
养基上经卡那霉素筛选，PCR、Southern 检测，结果得到5 个转基因阳性株系；组培苗抗寒试验结果表明，
相同低温（–4 和–8 ℃）条件下，转基因植株均比对照表现出较高的成活率；随着处理温度的降低，转
基因株系REC 与MDA 含量始终低于对照植株；转基因植株的低温半致死温度（LT50）比未转基因对照
低2.13 ℃。试验结果表明导入的AmEBP1 基因提高了‘大果’杏的抗寒性。
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| 关 键 词： | 杏 花粉管导入 AmEBP1 基因 基因转化 |
Molecular Identification and Cold-resistance Analysis of the AmEBP1 Transgenic Apricot Plants |
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| NIU Qing-Lin,CAO Bang-Hua,WANG Yu-Shan,LIU Jing,ZHAO Jin-Hong,LUO Lei,HUANG Yan-Yan,FENG Dian-Qi.Molecular Identification and Cold-resistance Analysis of the AmEBP1 Transgenic Apricot Plants[J].Acta Horticulturae Sinica,2014,41(5):825-831. |
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| Authors: | NIU Qing-Lin CAO Bang-Hua WANG Yu-Shan LIU Jing ZHAO Jin-Hong LUO Lei HUANG Yan-Yan FENG Dian-Qi |
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| Institution: | Department of Immunobiology, Jinan University, Guangzhou 510632, China. |
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| Abstract: | The AmEBP1 gene coming from Ammopiptanthus mongolicus，was transformed into the
immature embryo of apricot by pollen tube. Grew in the modified WPM media，five transgenic lines were
obtained based on the following selections including kanamycin resistance，PCR and Southern analyses.
Results of the cold resistance tests pointed out that in the low temperature treatments（–4 and–8 ℃），the
transgenic plants exhibited higher survival rates than the untransformed control plants. Under the lower
temperature cold treatment，the REC and MDA of transgenic plants was obviously lower than that of
untransformed control plants. The LT50 of transgenic lines was remarkable lower than that of the
untransformed control plants by 2.13 ℃. These results indicate that the imported AmEBP1 gene increased
the cold resistance of the Daguo apricot. |
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| Keywords: | apricot pollen tube AmEBP1 gene gene transformation |
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