| 结球甘蓝核基因组密码子使用偏爱性分析 |
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| 引用本文： | 仪登霞,杨丽梅,方智远,刘玉梅,庄 木,张扬勇,刘基生,刘 博,武 剑,耿丽丽,张 杰,张振贤.结球甘蓝核基因组密码子使用偏爱性分析[J].园艺学报,2013,40(10):1927-1934. |
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| 作者姓名： | 仪登霞 杨丽梅 方智远 刘玉梅 庄 木 张扬勇 刘基生 刘 博 武 剑 耿丽丽 张 杰 张振贤 |
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| 作者单位： | （1中国农业科学院蔬菜花卉研究所，北京100081；2中国农业科学院植物保护研究所，北京100193；3中国农业大学农学与生物技术学院，北京100193） |
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| 摘 要： | 根据甘蓝测序结果，利用CodonW软件对结球甘蓝的48 000个蛋白质编码基因序列进行了分析，计算出同义密码子相对使用频率，确定了TCT、CCT、AGA、GTT、GCT、GAT等6个高频密码子。将甘蓝与大肠杆菌、拟南芥、棉花、水稻的密码子使用频率进行了比较，发现甘蓝密码子使用偏爱性与同为双子叶植物的拟南芥、棉花基本一致，而与大肠杆菌及单子叶植物水稻均具有较大差异。以甘蓝密码子用法分析结果为依据，对Bt cry1C抗虫基因进行了密码子的改造，得到了具有甘蓝密码子使用特点的cry1C基因序列，为甘蓝的转基因研究奠定了分子基础。
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| 关 键 词： | 甘蓝 密码子用法 高频密码子 密码子偏爱性 |
| 收稿时间： | 2013-05-03 |
Analysis of Nuclear Genome Codon Preference in Cabbage |
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| YI Deng-xia,YANG Li-mei,FANG Zhi-yuan,LIU Yu-mei,ZHUANG Mu,ZHANG Yang-yong,LIU Ji-sheng,LIU Bo,WU Jian,GENG Li-li,ZHANG Jie,ZHANG Zhen-xian,.Analysis of Nuclear Genome Codon Preference in Cabbage[J].Acta Horticulturae Sinica,2013,40(10):1927-1934. |
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| Authors: | YI Deng-xia YANG Li-mei FANG Zhi-yuan LIU Yu-mei ZHUANG Mu ZHANG Yang-yong LIU Ji-sheng LIU Bo WU Jian GENG Li-li ZHANG Jie ZHANG Zhen-xian |
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| Institution: | （1Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China；2Institute of Plant Protection，Chinese Academy of Agricultural Sciences，Beijing 100193，China；3College of Agriculture and Biotechnology，China Agricultural University，Beijing 100193，China） |
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| Abstract: | Forty-eight thousand coding DNA sequences of cabbage were used to analyze the relative frequency of synonymous codon（RFSC）. Six high-frequency codons including TCT，CCT，AGA，GTT，GCT，and GAT were revealed by CodonW. When the frequency of codon usage of cabbage was compared to Escherichia coli，Arabidopsis thaliana，and Oryza sativa，we found that the codon preference was considerably different between cabbage and Escherichia coli，Oryza sativa，while the codon preference was identical between cabbage and Arabidopsis thaliana，Gossypium hirsutum. The codons of Bt cry1C gene sequence have been reconstructed according to the results. The foreigngene containing the optimal codons of cabbage is helpful to researches involving cabbage transformation. |
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| Keywords: | cabbage codon usage high-frequency condon condon preference |
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