| ‘月月红’月季体细胞胚胎发生和植株再生研究 |
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| 引用本文： | 易,星,陈己任,胡博文,陈彦斌,李,青,邓子牛,熊兴耀.‘月月红’月季体细胞胚胎发生和植株再生研究[J].园艺学报,2014,41(4):781-788. |
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| 作者姓名： | 易 星 陈己任 胡博文 陈彦斌 李 青 邓子牛 熊兴耀 |
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| 作者单位： | （1湖南农业大学园艺园林学院，长沙 410128；2中国农业科学院蔬菜花卉研究所，北京 100081） |
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| 摘 要： | 以建立月季高频再生体系为目标，以‘月月红’月季叶片为外植体，探索了生长调节剂和光照条件对其体细胞胚胎发生及其植株再生的影响。结果表明：3.0 mg · L-12,4-D + 0.5mg · L-1 TDZ的胚分化培养基上暗培养12周后转入胚成熟培养基，置于红光（光强约为7.2 µmol · m-2 · s-1）条件下培养，能产生较多具肥厚子叶且有高频再生潜力的体细胞胚。暗培养条件下主要产生无子叶的芽状胚。 胚性愈伤组织在含
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| 关 键 词： | 月季 红光 体细胞胚胎发生 器官发生 |
| 收稿时间： | 2013-12-20 |
Studies on Somatic Embryogenesis and Plant Regeneration in Rosa chinensis‘Yueyuehong’ |
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| YI Xing,CHEN Ji-ren,HU Bo-wen,CHEN Yan-bin,LI Qing,DENG Zi-niu,XIONG Xing-yao,.Studies on Somatic Embryogenesis and Plant Regeneration in Rosa chinensis‘Yueyuehong’[J].Acta Horticulturae Sinica,2014,41(4):781-788. |
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| Authors: | YI Xing CHEN Ji-ren HU Bo-wen CHEN Yan-bin LI Qing DENG Zi-niu XIONG Xing-yao |
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| Institution: | （1College of Horticulture and Gardening，Hunan Agricultural University，Changsha 410128，China；2Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China） |
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| Abstract: | In order to establish a high-frequency regeneration system for rose，the crucial effects of light and plant growth regulators on somatic embryogenesis and plant regeneration in Rosa chinensis ‘Yueyuehong’were investigated using leaf as explant. The results showed that after 12 weeks of callus induction on embryo proliferation medium supplemented with 3.0 mg · L-1 2,4-D + 0.5mg · L-1 TDZ in the dark and a subculture on embryo mature medium under red light（with an intensity of 7.2 µmol · m-2 · s-1）treatment，somatic embryos with thick and expanded cotyledon（s），which had potential regenerating capability were mostly observed. While shoot-like embryos without cotyledon（s）were mostly observed in dark condition. |
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| Keywords: | rose red light somatic embryogenesis organogenesis |
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