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| 砧用南瓜种质资源形态学性状与SSR标记分析 | |
| 引用本文： | 李鹤,郭世荣,束胜,徐扬,孙锦.砧用南瓜种质资源形态学性状与SSR标记分析[J].园艺学报,2014,41(7):1379-1390. |
| 作者姓名： | 李鹤 郭世荣 束胜 徐扬 孙锦 |
| 作者单位： | 1 南京农业大学园艺学院，农业部南方蔬菜遗传改良重点开放实验室，江苏省现代设施农业技术与装备工程实验室， 南京 210095；2 南京农业大学（宿迁）设施园艺研究院，江苏宿迁 223800 |
| 基金项目： | 国家现代农业产业技术体系建设专项资金项目（CARS-25-C-03）；江苏省农业三新工程项目（SXGC[2012]391） |
| 摘 要： | 搜集了设施黄瓜嫁接栽培中常用的47 份砧用南瓜种质资源，利用63 个形态标记和40 对南
瓜SSR 标记，对其进行遗传多样性及亲缘关系鉴定。结果表明，砧用南瓜的8 个质量性状（嫩瓜皮色、
瓜形、主蔓色、瓜面斑纹、叶面白斑、叶形、嫩瓜斑纹和嫩瓜斑纹色）和23 个数量性状的多样性指数大
于1.00；SSR 标记共检测到167 个等位变异，平均每个SSR 位点的等位基因数为4.18 个，变幅2 ~ 8 个，
平均多样性指数为0.62，变化范围0.16 ~ 1.18。非加权算术平均法（UPGMA）聚类分析表明，砧用南瓜
63 个形态学性状将47 份种质划分为4 个类群，大部分品种集中于一个类群中；40 对多态性SSR 标记将
所有种质划分为3 个类群，大部分品种的分子标记与形态学标记的聚类结果相似。已搜集的47 份砧用南
瓜种质资源遗传多样性并不丰富，亲缘关系较近；SSR 标记CMTm11 具有极高的多态性，可用于砧用南
瓜种质分子标记辅助选择；嫩瓜皮色等8 个质量性状和大部分数量性状都可以作为形态标记辅助种质资
源评价及新品种选育。 |
| 关 键 词： | 南瓜 砧木 形态学 SSR |
Germplasm Resources Analysis of Rootstock-used Pumpkins by Phenotype and SSR |
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| LI He,GUO Shi-Rong,SHU Sheng,XU Yang,SUN Jin.Germplasm Resources Analysis of Rootstock-used Pumpkins by Phenotype and SSR[J].Acta Horticulturae Sinica,2014,41(7):1379-1390. | |
| Authors: | LI He GUO Shi-Rong SHU Sheng XU Yang SUN Jin |
| Institution: | 1College of Horticulture，Nanjing Agricultural University，Key Laboratory of Southern Vegetables Genetic Improvement of Ministry of Agriculture，Nanjing 210095，China； 2 Facility Horticulture Institute，Nanjing Agricultural University，Suqian， Jiangsu 223800，China |
| Abstract: | Forty-seven accessions of rootstock-used pumpkin were collected in this research. Analyses of genetic diversity and relationships were done with 63 phenotypes and 40 SSR markers. It was observed that H’> 1.00 with eight qualitative characters（tender gourd skin color，fruit shape，main vine color， skin speckle，foliage white spotting，leaf shape，tender gourd speckle and color）and 23 quantitative characters. One hundred and sixty-seven polymorphic bands were generated. An average of 4.18 polymorphic bands were detected from one pair SSR primers，and they ranged from 2 to 8. H’ranged from 0.16 to 1.18，and its average was 0.62. Forty-seven germplasms were divided into four groups by 63 morphological traits，and most of the germplasms were clustered into one group. Three groups were divided by 40 higher polymorphic SSR primers，similar to the result of our previous morphological study. The genetic diversity of collected rootstock-used pumpkin germplasms was low，and the genetic relationship was close. SSR marker CMTm11，with a high polymorphism，could be used in molecular marker assisted selection. Eight quality traits and most of the quantitative traits could be used as morphological markers， as an auxiliary means of rootstock-used pumpkin germplasms evaluation and breeding of new varieties. |
| Keywords: | pumpkin rootstock phenotype SSR |
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