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| 柑橘CsTBL1启动子的克隆及其在转基因拟南芥中的活性分析 | |
| 引用本文： | 马岩岩,陈 娇,吴天利,张 军,钟广炎.柑橘CsTBL1启动子的克隆及其在转基因拟南芥中的活性分析[J].园艺学报,2014,41(5):817-824. |
| 作者姓名： | 马岩岩 陈 娇 吴天利 张 军 钟广炎 |
| 作者单位： | 1 西南大学园艺园林学院/中国农业科学院柑桔研究所，重庆 400715；2 农业部南亚热带果树生物学和遗传资源利用重点实验室/广东省农业科学院果树研究所，广州 510640 |
| 基金项目： | 农业部公益性行业（农业）科研专项（201203075-07）；重庆市科技攻关计划项目（CSTTC，2007AA1018） |
| 摘 要： | 以奥林达夏橙Citrus sinensis(L.)‘Olinda’]为材料,克隆了CsTBL1基因编码起始位点上游长度为2 361 bp的启动子序列。生物信息学预测表明,该启动子中含有多个与逆境应答相关的顺式作用元件。为进一步分析CsTBL1启动子的功能,构建了该基因启动子与GUS基因融合的植物表达载体,并用浸花法转化拟南芥,对T3代转基因拟南芥进行GUS活性染色。结果显示,CsTBL1启动子在1～3 d龄幼苗所有组织中的活性都非常强,在7～20 d龄幼苗的子叶和根中的活性仍然较强,但在下胚轴中基本检测不到活性。在50 d龄幼苗中,只在叶、茎的毛状体以及还在生长的根中检测到GUS活性。转基因植株的GUS表达受到1–氨基环丙烷–1–羧酸(乙烯合成前体,ACC)、脱落酸(ABA)、甲基茉莉酸(MeJA)和水杨酸(SA)诱导。上述结果表明CsTBL1可能在植物发育和抗外界逆境胁迫中起到非常重要的作用。 |
| 关 键 词： | 柑橘 启动子 GUS 基因 CsTBL1 基因 |
Isolation of the Citrus CsTBL1 Gene Promoter and Its Activity in Transgenic Arabidopsis |
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| MA Yan-Yan,CHEN Jiao,WU Tian-Li,ZHANG Jun,ZHONG Guang-Yan.Isolation of the Citrus CsTBL1 Gene Promoter and Its Activity in Transgenic Arabidopsis[J].Acta Horticulturae Sinica,2014,41(5):817-824. | |
| Authors: | MA Yan-Yan CHEN Jiao WU Tian-Li ZHANG Jun ZHONG Guang-Yan |
| Institution: | 1College of Horticulture and Landscape Architecture，Southwest University/Citrus Research Institute，Chinese Academy of Agricultural Sciences，Chongqing 400715，China；2Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization，Ministry of Agriculture/Institution of Fruit Tree Research，Guangdong Academy of Agricultural Sciences，Guangzhou 510640，China |
| Abstract: | A 2 361 bp long promoter of the CsTBL1 gene was isolated from sweet orangeCitrus sinensis（L.）‘Olinda’]. Sequence analysis showed that the promoter harbors multiple stress-responsive cis-elements. To gather clues of the specific functions of the promoter a promoter-reporter vector was constructed，and introduced it into Arabidopsis by floral dip method. Analysis of T3 transgenic progenies with histochemical staining showed that high GUS activity in whole plants of 1- and 3-day-old seedlings. Strong GUS activity was still detected in cotyledons and roots of 7- and 20-day-old seedlings，but disappeared in hypocotyls. In 50-day-old seedlings，however，GUS activity was only detected in root tips and trichomes. GUS expression was induced by treatments with ethylene precursor ACC，ABA，MeJA，and SA，suggesting that CsTBL1 could be involved in plant development and stress responses. |
| Keywords: | citrus promoter GUS gene CsTBL1 gene |
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