| 以柑橘多胚性二倍体母本倍性杂交培育三倍体 |
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| 引用本文： | 解凯东,王晓培,王惠芹,梁武军,谢宗周 郭大勇,伊华林,邓秀新,Grosser Jude W. 郭文武.以柑橘多胚性二倍体母本倍性杂交培育三倍体[J].园艺学报,2014,41(4):613-620. |
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| 作者姓名： | 解凯东 王晓培 王惠芹 梁武军 谢宗周 郭大勇 伊华林 邓秀新 Grosser Jude W. 郭文武 |
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| 作者单位： | （1华中农业大学园艺植物生物学教育部重点实验室/农业部华中地区园艺作物生物学与种质创制重点实验室，武汉 430070；2 美国佛罗里达大学柑橘研究与教育中心，佛罗里达 33850，美国） |
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| 摘 要： | 2009—2012年,以柑橘8个多胚性二倍体品种为母本与近年始花的5个异源四倍体体细胞杂种和1个同源四倍体分别杂交,培育三倍体新种质。连续4年共配置15个杂交组合,授粉4 442朵花,坐果1 484个,平均坐果率33.4%;用于幼胚挽救的果实1 075个,共培养幼嫩种子12 578颗,经生芽、生根诱导获得再生植株2 832个;以流式细胞仪对所有再生植株倍性检测,15个组合共获得三倍体401个,四倍体121个;移栽成活三倍体349个,四倍体98个。对W.默科特×NH组合89个株系的三倍体的SSR分子鉴定表明,所有三倍体后代均为双亲的有性杂种。
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| 关 键 词： | 柑橘 三倍体 有性杂交 倍性分析 SSR分子标记 |
| 收稿时间： | 2013-09-30 |
High Efficient and Extensive Production of Triploid Citrus Plants by Crossing Polyembryonic Diploids with Tetraploids |
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| XIE Kai-dong,WANG Xiao-pei,WANG Hui-qin,LIANG Wu-jun,Xie Zong-zhou,GUO Da-yong,YI Hua-lin,DENG Xiu-xin,GROSSER Jude W.,GUO Wen-wu,.High Efficient and Extensive Production of Triploid Citrus Plants by Crossing Polyembryonic Diploids with Tetraploids[J].Acta Horticulturae Sinica,2014,41(4):613-620. |
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| Authors: | XIE Kai-dong WANG Xiao-pei WANG Hui-qin LIANG Wu-jun Xie Zong-zhou GUO Da-yong YI Hua-lin DENG Xiu-xin GROSSER Jude W GUO Wen-wu |
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| Institution: | （1Key Laboratory of Horticultural Plant Biology（Ministry of Education）/Key Laboratory of Central China Horticultural Crop Biology and Germplasm Enhancement（Ministry of Agriculture），Huazhong Agricultural University，Wuhan 430070，China；2Citrus Research and Education Center，University of Florida，Lake Alfred，FL 33850，USA） |
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| Abstract: | In the four successive years from 2009 to 2012，to obtain triploid citrus hybrids，eight elite polyembryonic diploid female parents were crossed respectively with five allotetraploid somatic hybrids and one autotetraploid. A total of 4 442 flowers were pollinated in 15 crosses，from which 1 484 fruits were set，with an average fruit setting rate as 33.4%. As much as 12 578 immature seeds from 1 075 young fruits were cultured in vitro. After shooting and rooting induction，2 832 plants were regenerated，in which 401 and 121 plants proved to be triploids and tetraploids respectively by flow cytometry analysis. Up to 349 triploids and 98 tetraploids survived in the greenhouse after transplantation into greenhouse. The Simple sequence repeat（SSR）markers analysis showed that all of the 89 triploids derived from the cross of W. Murcott × NH were hybrids of both parents. |
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| Keywords: | citrus triploid interploid hybridization ploidy analysis SSR marker |
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