| 抗菌肽基因Gnk2-1经花粉管通道法导入西瓜的初步研究 |
| |
| 引用本文： | 许珺然,张,显,张,勇,马建祥.抗菌肽基因Gnk2-1经花粉管通道法导入西瓜的初步研究[J].园艺学报,2014,41(7):1467-1475. |
| |
| 作者姓名： | 许珺然 张 显 张 勇 马建祥 |
| |
| 作者单位： | 西北农林科技大学园艺学院，陕西杨凌 712100 |
| |
| 基金项目： | 国家自然科学基金项目（31372086） |
| |
| 摘 要： | 通过花粉管通道介导银杏抗菌肽基因Gnk2-1 于西瓜自交系‘04-1-2’中，以浓度为100、200、
300 和400 ng · μL-1 的DNA 为供体，分别于授粉后24、27、30 和33 h 导入，对子代进行PCR 检测。结
果表明：T0 代坐果率和单瓜结籽数随授粉后处理时间的增加而升高，但在各个处理时间下均显著低于对
照；外源DNA 的导入对授粉后27 h 处理的T1 代种子发芽率和授粉后24 h 处理的T1 代种子出苗率均造成
显著降低；不同DNA 浓度转化的T1 代种子的发芽率和出苗率与对照相比差异不显著；对1 280 株T1 幼
苗进行PCR 检测，得到32 株阳性转化植株，总体转化率为2.5%，最佳转化时间为授粉后24 ~ 27 h，最佳
DNA 转化浓度为100 ~ 200 ng · μL-1；PCR 阳性植株抗病鉴定表明转基因植株对西瓜枯萎病的抗性有所增强。
|
| 关 键 词： | 西瓜 花粉管通道法 分子检测 抗菌肽 |
Introduction of Antimicrobial Peptide Gene Gnk2-1 into Watermelon via
Pollen-tube Pathway |
| |
| XU Jun-Ran,ZHANG,XIAN,ZHANG,YONG,MA Jian-Xiang.Introduction of Antimicrobial Peptide Gene Gnk2-1 into Watermelon via
Pollen-tube Pathway[J].Acta Horticulturae Sinica,2014,41(7):1467-1475. |
| |
| Authors: | XU Jun-Ran ZHANG XIAN ZHANG YONG MA Jian-Xiang |
| |
| Institution: | College of Horticulture，Northwest A & F University，Yangling，Shaanxi 712100，China |
| |
| Abstract: | In order to study the feasibility of transferring exogenous DNA into watermelon by
pollen-tube pathway，the antimicrobial peptide gene Gnk2-1 was introduced into watermelon accession
‘04-1-2’by pollen-tube pathway with different concentration（100，200，300 and 400 ng · μL-1）at different
time（24 h，27 h，30 h，33 h）after self-pollination. Seeds from treated fruits were harvested and tested by
PCR. The results were as follows：The fruit setting rate and average number of seeds of T0 generation
increased with the increment of treatment time after self-pollination，though the two figures both showed a
significant decrease under all treatments compared with control. The germination rate of T1 seeds treated
27 h after self-pollination and the seedling emergence rate of T1 seeds treated 24 h after self-pollination
decreased significantly. Compared with control，there was no distinct difference illustrated in the rate of
germination and seedling emergence of T1 seeds between different concentration treatments. The PCR
results of 1 280 tested T1 seedlings showed 32 positive plants and the total transformation rate was 2.5%.
The optimum transformation time was 24–27 h after self-pollination and the optimum transformation concentration were 100–200 ng · μL-1. The positive plants were inoculated with Fusarium and showed a
stronger resistance compared with non transgenic plants. |
| |
| Keywords: | watermelon pollen-tube pathway molecular detection antimicrobial peptide |
|
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
