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野生毛花猕猴桃叶片和果实AsA 含量的SSR 标记关联分析
引用本文:汤佳乐,吴 寒,郎彬彬,曲雪艳,黄春辉,徐小彪.野生毛花猕猴桃叶片和果实AsA 含量的SSR 标记关联分析[J].园艺学报,2014,41(5):833-840.
作者姓名:汤佳乐  吴 寒  郎彬彬  曲雪艳  黄春辉  徐小彪
作者单位:江西农业大学农学院,南昌 330045
基金项目:国家自然科学基金项目(31360472);江西省自然科学基金项目(20132BAB204025)
摘    要: 以抗坏血酸(AsA)含量变异丰富的野生毛花猕猴桃(Actinidia eriantha Benth.)种群为试 验材料,对其叶片和果实中的AsA 含量与SSR 标记进行关联分析。结果表明:通过SSR 分子标记技术和 群体结构分析,可将70 份野生毛花猕猴桃种质材料分为4 个亚群;GLM 模型分析结果显示,在P < 0.01 水平上检测有7 个SSR 标记与叶片和果实AsA 含量紧密相关,各标记对叶片和果实AsA 含量变异的解释 率在0.082 5 ~ 0.175 5;MLM 模型分析结果显示,在P < 0.01 的水平上共检测到有6 个SSR 标记分别与 叶片和果实AsA 含量紧密相关,各个标记的解释率在0.073 6 ~ 0.154 6 之间。发现了与叶片及果实中的 AsA 含量关联的优异SSR 标记位点(UDK96-040、UDK96-053、UDK97-408、UDK97-414 和Ke227)。

关 键 词:毛花猕猴桃  抗坏血酸  群体结构分析  关联分析

Association Analysis on Leaf and Fruit AsA Content and SSR Markers of Wild Actinidia eriantha
TANG Jia-Le,WU Han,LANG Bin-Bin,QU Xue-Yan,HUANG Chun-Hui,XU Xiao-Biao.Association Analysis on Leaf and Fruit AsA Content and SSR Markers of Wild Actinidia eriantha[J].Acta Horticulturae Sinica,2014,41(5):833-840.
Authors:TANG Jia-Le  WU Han  LANG Bin-Bin  QU Xue-Yan  HUANG Chun-Hui  XU Xiao-Biao
Institution:College of Agronomy,Jiangxi Agricultural University,Nanchang 330045,China
Abstract:Association analysis on leaf and fruit AsA content and SSR markers were studied by using wild Actinidia eriantha Benth. populations which AsA content was rich variation as experimental material. The results indicated that by using SSR marker and genetic structure analysis showed that the population of these materials was composed of 4 subpopulations. At P < 0.01 level,there were 7 SSR markers associated with the leaf and fruit AsA content under GLM program,and the rate of explanation on the phenotype of related marker ranged from 0.082 5 to 0.175 5. There were 6 SSR markers associated with the leaf and fruit AsA content under MLM program,and the rate of explanation on the phenotype of related marker ranged from 0.073 6 to 0.154 6. Found excellent SSR markers(UDK96-040,UDK96-053, UDK97-408,UDK97-414 and Ke227)associated with the leaf and fruit AsA content.
Keywords:Actinidia eriantha  ascorbic acid(AsA)  population structure analysis  association analysis
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