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| 辣椒种间（Capsicum annuum × C. frutescens）遗传图谱的构建与分析 | |
| 引用本文： | 周坤华,陈学军,方 荣,陈丽珍,宗洪霞,缪南生.辣椒种间（Capsicum annuum × C. frutescens）遗传图谱的构建与分析[J].园艺学报,2013,40(11):2171-2179. |
| 作者姓名： | 周坤华 陈学军 方 荣 陈丽珍 宗洪霞 缪南生 |
| 作者单位： | 1 江西省农业科学院蔬菜花卉研究所，南昌 330200； 2 江西省农业科学院油料作物重点实验室，南昌 330200；3 江西省农业科学院，南昌 330200；4 江西农业大学农学院，南昌 330045 |
| 基金项目： | 国家自然科学基金项目（30860173，31060203） |
| 摘 要： | 以一年生辣椒（Capsicum annuum）B9431 为母本（P1），中国野生灌木辣椒（C. frutescens）
H108 为父本（P2）进行种间杂交，得到包含180 个单株的F2 作图群体，利用SRAP、SSR、ISSR 标记和
形态标记构建辣椒遗传图谱。该图谱共包含14 个连锁群，涉及264 个SRAP 标记、32 个SSR 标记和2
个ISSR 标记，控制软肉落果性状的S 基因定位于LG8。图谱总长1 023.45 cM，标记间平均图距3.42 cM。
每个连锁群的标记数在2 ~ 44 个之间，连锁群的长度在13.84 ~ 129.93 cM 范围内，平均图距在2.38 ~ 12.90
cM 之间。以SSR 标记为锚定标记，将该图谱与Minamiyama 等构建的图谱进行了初步对应。 |
| 关 键 词： | 辣椒 种间遗传图谱 SRAP SSR ISSR |
Construction and Analysis of an Interspecific Linkage Map of Capsicum annuum × C. frutescens |
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| Institution: | 1Vegetable and Flower Institute，Jiangxi Academy of Agricultural Sciences，Nanchang 330200，China；2The Key Laboratory of Oil Crop，Jiangxi Academy of Agricultural Sciences，Nanchang 330200，China；3Jiangxi Academy of Agricultural Sciences，Nanchang 330200，China；4College of Agriculture，Jiangxi Agricultural University，Nanchang 330045，China |
| Abstract: | In this study，a population of F2 generation including 180 individual plants was derived from the interspecific cross between the female line Capsicum annuum B9431 and the male line C. frutescens H108. The SRAP，SSR，ISSR markers and morphological marker were used in constructing a genetic map. A genetic linkage map containing 14 linkage groups（LGs）was constructed using 264 SRAP，32 SSR and 2 ISSR. The S gene controlling the soft flesh and deciduous fruit was located at LG8. The map covered 1 023.45 cM with an average marker interval of 3.42 cM. Number of markers included in linkage groups varied from 2 to 44，mean marker interval distance from 2.38 cM to 12.90 cM and the length of linkage groups from 13.84 cM to 129.93 cM individually. Furthermore，the links between the map published by Minamiyama et al. and ours were established by the public microsatellites（SSR markers）. |
| Keywords: | pepper interspecific genetic map SRAP SSR ISSR |
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