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| 培养基中糖和氮对脱毒甘薯组培苗生长的影响 | |
| 引用本文： | 王守明,胥学峰,廉美兰,朴炫春,朴永昱.培养基中糖和氮对脱毒甘薯组培苗生长的影响[J].延边大学农学学报,2006,28(2):102-106. |
| 作者姓名： | 王守明 胥学峰 廉美兰 朴炫春 朴永昱 |
| 作者单位： | 1. 延边大学农学院,园艺系,吉林,龙井,133400 2. 吉林农业科技学院,吉林,吉林,132101 3. 延吉市园林处,吉林,延吉,133000 |
| 基金项目： | 延大科合字（2003）第08号. |
| 摘 要： | 以脱毒甘薯的茎段为材料，研究培养基中不同糖种类、糖浓度、NH4^＋/NO3^-及总氮量对甘薯组培苗扩繁的影响．结果表明：在MS培养基添加蔗糖或白糖，NH4^＋/NO3^-含量为30／30（mM／mM），总氮量控制在30，60mM时对甘薯组培扩繁有利．考虑到培养基成本，脱毒甘薯培养基以MS＋食用白糖50g／L＋NH4^＋/NO3^-30／30（mM／mM）＋总氮量30mM最为适宜．将脱毒甘薯组培苗移栽到含蛭石和草炭的营养钵中进行驯化，成活率可达95％． |
| 关 键 词： | 甘薯 糖种类 糖浓度 总氮量 |
| 文章编号： | 1004-7999(2006)02-0102-05 |
| 收稿时间： | 2006-03-25 |
| 修稿时间： | 2006年3月25日 |
Effects of sugar and nitrogen on virus-free plantlet growth of sweet potato in vitro |
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| WANG Shou-ming,XU Xue-feng,LIAN Mei-lan,PIAO Xuan-chun,PIAO Yong-yu.Effects of sugar and nitrogen on virus-free plantlet growth of sweet potato in vitro[J].Journal of Agricultural Science Yanbian University,2006,28(2):102-106. | |
| Authors: | WANG Shou-ming XU Xue-feng LIAN Mei-lan PIAO Xuan-chun PIAO Yong-yu |
| Institution: | 1. Department of Horticulture, Agricultural College of Yanbian University, Longjing Jilin 133400, China ; 2. Agricultural Scientific and Technical College ,Jilin 132101, China ; 3. Landscape Administrative Office, Yanji Jilin 133000, China |
| Abstract: | The effects of different types of sugar,sugar with different concentrations,(NH~+_4/NO~-_3,)and total nitrogen in culture medium on the micropropagation of sweet potatoes were studied with nodal cuttings of virus-free plantlet.The results showed that the plantlet growth was favored in the 50 g/L or 70 g/L sucrose or white sugar,30/30(mM /mM) NH~+_4/NO~-_3,and 30 mM or 60 mM total nitrogen concentration.Considering the medium cost,the medium of MS+ 50 g/L white sugar +30/30(mM /mM)NH~+_4/NO~-_3 +30 mM total nitrogen was optimal for sweet potato growth in vitro.During the acclimatization stage,the survival rate could reach 95% when the plantlets were transferred in pots containing vermiculite and peat. |
| Keywords: | NH4 /NO3- |
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