| 珠三角地区猪圆环病毒2型的分离鉴定及序列分析 |
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| 引用本文： | 黎丁滔,陈芳艳,陈瑞爱,晏勇邦,冯金牛,王林川.珠三角地区猪圆环病毒2型的分离鉴定及序列分析[J].动物医学进展,2011,32(5):5-9. |
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| 作者姓名： | 黎丁滔 陈芳艳 陈瑞爱 晏勇邦 冯金牛 王林川 |
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| 作者单位： | 黎丁滔,王林川,LI Ding-tao,WANG Lin-chuan(华南农业大学兽医学院,广东,广州,510642;广东大华农动物保健品股份有限公司,广东新兴,527400);陈芳艳,CHEN Fang-yan(华南农业大学动物科技学院,广东,广州,510642);陈瑞爱,CHEN Rui-ai(广东大华农动物保健品股份有限公司,广东新兴,527400);晏勇邦,冯金牛,YAN Yong-bang,FENG Jin-niu(华南农业大学兽医学院,广东,广州,510642) |
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| 摘 要： | 为了解珠三角地区猪群中猪圆环病毒2型(PCV-2)的流行变异情况,从2008年-2010年珠三角地区疑似PMWS病死猪组织中分离获得PCV-2流行毒株,用间接免疫荧光方法进行检测和用PCR方法对这些毒株进行全基因扩增及测序分析.结果显示,在接毒细胞内观察到了特异性免疫荧光,8株PCV-2分离株与GenBank中的其他P...
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| 关 键 词： | 猪圆环病毒2型 分离 鉴定 序列分析 |
Isolation,Identification and Sequence Analysis of Porcine Circovirus Type 2 from Pigs in Pearl River Delta Area |
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| LI Ding-tao,CHEN Fang-yan,CHEN Rui-ai,YAN Yong-bang,FENG Jin-niu,WANG Lin-chuan.Isolation,Identification and Sequence Analysis of Porcine Circovirus Type 2 from Pigs in Pearl River Delta Area[J].Progress In Veterinary Medicine,2011,32(5):5-9. |
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| Authors: | LI Ding-tao CHEN Fang-yan CHEN Rui-ai YAN Yong-bang FENG Jin-niu WANG Lin-chuan |
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| Institution: | LI Ding-tao1,2,CHEN Fang-yan3,CHEN Rui-ai2,YAN Yong-bang1,FENG Jin-niu1,WANG Lin-chuan1,2(1.College of Animal Science,South China Agricultural University,Guangzhou,Guangdong,510642,China,2.Guangdong Huanong-wens Animal Husbandry co.ltd,Xinxing,527400,3.College of Veterinary Science,China) |
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| Abstract: | In order to get the message of the epidemiology and sequence variation of the porcine circovirus type 2(PCV-2),strains of PCV-2 were isolated from the died pigs associated with post-weaning multisystemic wasting syndrome(PWMS) in Pearl River Delta area.The PCV-2 nucleic acid fragments were detected from the infected PK-15 cell and the complete genome of eight PCV-2 isolates were amplified and sequenced.The results indicated the specific PCV-2 antigen were shown through immunofluorescence in the infected cyt... |
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| Keywords: | Porcine circovirus type 2 isolation identification sequence analysis |
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