| 猪圆环病毒2型四川分离株ORF2基因的克隆与序列分析 |
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| 引用本文： | 杨泽晓,刘宁,王印,姚学萍,张彦明,杨增岐,汪开毓,韩茜.猪圆环病毒2型四川分离株ORF2基因的克隆与序列分析[J].动物医学进展,2011,32(6). |
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| 作者姓名： | 杨泽晓 刘宁 王印 姚学萍 张彦明 杨增岐 汪开毓 韩茜 |
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| 作者单位： | 杨泽晓,YANG Ze-xiao(四川农业大学动物医学院,四川雅安,625014;动物疫病与人类健康四川省重点实验室,四川雅安,625014);刘宁,LIU Ning(四川省草业科学研究院,四川新津,611430);王印,姚学萍,汪开毓,韩茜,WANG Yin,YAO Xue-ping,WANG Kai-yu,HAN Xi(四川农业大学动物医学院,四川雅安,625014);张彦明,杨增岐,ZHANG Yan-ming,YANG Zeng-qi(西北农林科技大学动物医学院,陕西杨陵,712100) |
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| 基金项目： | 教育部《长江学者和创新团队发展计划》创新团队项目，四川农业大学双支计划 |
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| 摘 要： | 对临床分离毒株PCV-2 sch2010 ORF2基因全序列进行了克隆和序列分析.结果表明,PCV-2sch2010 ORF2全基因共705 bp,编码234个氨基酸,与GenBank发表的21株PCV-2的ORF2参考序列的核苷酸氨基酸序列同源性分别为88.4%～99.7%和87.2%～99.6%;与2株PCV-1 ORF2(GU371908、FJ475129)的核苷酸序列和氨基酸序列同源性分别为58.7%～59.5%和62.8%～64.5%;PCV-2 sch2010分离株与PCV-2的欧洲分支群亲缘关系较近.
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| 关 键 词： | 猪圆环病毒2型 序列分析 |
Cloning and Sequence Analysis of the ORF2 Gene of PCV-2 Sichuan Isolate |
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| YANG Ze-xiao,LIU Ning,WANG Yin,YAO Xue-ping,ZHANG Yan-ming,YANG Zeng-qi,WANG Kai-yu,HAN Xi.Cloning and Sequence Analysis of the ORF2 Gene of PCV-2 Sichuan Isolate[J].Progress In Veterinary Medicine,2011,32(6). |
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| Authors: | YANG Ze-xiao LIU Ning WANG Yin YAO Xue-ping ZHANG Yan-ming YANG Zeng-qi WANG Kai-yu HAN Xi |
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| Institution: | YANG Ze-xiao1,4,LIU Ning3,WANG Yin1,YAO Xue-ping1,ZHANG Yan-ming2,YANG Zeng-qi2,WANG Kai-yu1,HAN Xi1(1.College of Veterinary Medicine,Sichuan Agricultural University,Yaan Sichuan,625014,China,2.College of Veterinary Medicine,Northwest A& F University,Yangling Shaanxi,712100,3.Sichuan Pratacultural Science Research Institute,Xinjin,Sichuan,611430,4.Key laboratory of Animal Disease and Human Health of Sichuan Province,Yaan,China) |
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| Abstract: | To study the genetic heterogeneity and origin of PCV-2 in Sichuan province,the whole ORF2 gene of PCV-2 sch2010 isolated from the swine with clinical manifestation in Sichuan province was amplified and cloned into pGM-T vector,and analyzed with PCV ORF2 gene published in GenBank by using DNA Star software.The results showed ORF2 gene of PCV-2sch2010 encodes a fragment of 705 bp which coding 234 amino acids,and the genetic variation analysis indicated the ORF2 gene of PCV-2 Sichuan isolate had homdogies rang... |
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| Keywords: | ORF2 |
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