| 猴B病毒囊膜蛋白gC基因密码子优化及其在大肠埃希菌中的表达 |
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| 引用本文： | 王代平,叶华虎,曾林,白杰英,董罡,张文韬,聂奎.猴B病毒囊膜蛋白gC基因密码子优化及其在大肠埃希菌中的表达[J].动物医学进展,2011,32(5):1-4. |
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| 作者姓名： | 王代平 叶华虎 曾林 白杰英 董罡 张文韬 聂奎 |
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| 作者单位： | 王代平,张文韬,WANG Dai-ping,ZHANG Wen-tao(西南大学动物科技学院,重庆,400715;军事医学科学院实验动物中心,北京,100071);叶华虎,曾林,白杰英,董罡,YE Hua-hu,ZENG Lin,BAI Jie-ying,DONG Gang(军事医学科学院实验动物中心,北京,100071);聂奎,NIE Kui(西南大学动物科技学院,重庆,400715) |
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| 基金项目： | 军事医学科学院重大创新项目 |
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| 摘 要： | 根据猴B病毒E2490标准株gC基因,选择大肠埃希菌偏好的密码子,设计合成了1 200个碱基,共编码400个氨基酸,测序证实后,克隆入pET-28b(+)载体,转化至大肠埃希菌BL21(DE3),在IPTG诱导下获得50 ku以包涵体形式表达的gC重组蛋白.Western blot分析表明表达产物能够被猴B病毒标准阳性...
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| 关 键 词： | 猴B病毒 C糖蛋白 基因合成 高效表达 |
Optimization and Prokaryotic Expession of Gene Codon of Monkey B Virus Glycoprotein C |
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| WANG Dai-ping,YE Hua-hu,ZENG Lin,BAI Jie-ying,DONG Gang,ZHANG Wen-tao,NIE Kui.Optimization and Prokaryotic Expession of Gene Codon of Monkey B Virus Glycoprotein C[J].Progress In Veterinary Medicine,2011,32(5):1-4. |
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| Authors: | WANG Dai-ping YE Hua-hu ZENG Lin BAI Jie-ying DONG Gang ZHANG Wen-tao NIE Kui |
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| Institution: | 1(1.College of Animal Science and Technology,Southwest University,Chongqing,400715,China;2.Laboratory Animal Center of the Academy of Military Medical Sciences,Beijing,100071,China) |
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| Abstract: | In accordance with the gene sequence of E2490 of Monkey B virus standard strains,the biased codon in E.coli was selected.The complete length of the synthesis gene was 1200bp,which encoded 400 amino acids.The constructed gene fragment was cloned into pET-28b(+),and transformed into E.coli BL21.The culture was induced by IPTG.The recombinant protein was mainly located in the inclusion body.The Western blot showed the protein could react specifically with the monkey B virus positive serum.The indirect ELISA showed no cross reactivity with sera of human herpes simplex Ⅰ,Ⅱ positive sera.The laid the foundation for make the establishment of indirect ELISA for the detection of monkey B virus and the development of DNA vaccine. |
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| Keywords: | Monkey B virus glycoprotein C gene synthesis high expression |
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