| 鹅源H9N2亚型禽流感病毒PB2和PB1基因序列分析 |
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| 引用本文： | 付薇,覃芳芸,马琳,徐贤坤,黄胜斌,易春华,孙翔翔,何奇松,蒋家霞,熊毅.鹅源H9N2亚型禽流感病毒PB2和PB1基因序列分析[J].动物医学进展,2011,32(5):49-53. |
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| 作者姓名： | 付薇 覃芳芸 马琳 徐贤坤 黄胜斌 易春华 孙翔翔 何奇松 蒋家霞 熊毅 |
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| 作者单位： | 付薇,覃芳芸,马琳,徐贤坤,黄胜斌,熊毅,FU Wei,QIN Fang-yun,MA Lin,XU Xian-kun,HUANG Sheng-bin,XIONG Yi(广西动物疫病预防控制中心,广西南宁,530001);易春华,孙翔翔,何奇松,蒋家霞,YI Chun-hua,SUN Xiang-xiang,HE Qi-song,JIANG Jia-xia(广西大学动物科学技术学院,广西南宁,530005) |
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| 摘 要： | 采用RT-PCR方法对1株鹅源H9N2亚型禽流感病毒(GS/GX/01/07)RNA聚合酶基因PB2和PB1分别进行了扩增,将其克隆到pMD18-T载体后进行序列测定与分析.结果表明,该株病毒的PB2、PB1开放阅读框(ORF)分别由2 280和2 277个碱基组成,分别编码759和758个氨基酸.经同源性和系统进化树...
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| 关 键 词： | 禽流感病毒 H9N2亚型 聚合酶基因 序列分析 |
Cloning and Sequence Analysis of PB2 and PB1 Genes of H9N2 Avian Influenza Virus Strain Isolated from Guangxi Goose |
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| FU Wei,QIN Fang-yun,MA Lin,XU Xian-kun,HUANG Sheng-bin,YI Chun-hua,SUN Xiang-xiang,HE Qi-song,JIANG Jia-xia,XIONG Yi.Cloning and Sequence Analysis of PB2 and PB1 Genes of H9N2 Avian Influenza Virus Strain Isolated from Guangxi Goose[J].Progress In Veterinary Medicine,2011,32(5):49-53. |
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| Authors: | FU Wei QIN Fang-yun MA Lin XU Xian-kun HUANG Sheng-bin YI Chun-hua SUN Xiang-xiang HE Qi-song JIANG Jia-xia XIONG Yi |
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| Institution: | 1.Guangxi Center for Animal Disease Prevention and Control,Nanning,Guangxi,530001,China;2.College of Animal Science and Technology,Guangxi University,Nanning,Guangxi,530005,China) |
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| Abstract: | Full-length cDNAs of polymerase genes PB2 and PB1 of avian influenza virus strain GS/GX/01/06 were amplified by RT-PCR.The cDNAs were cloned into pMD18-T vectors and sequenced.Sequence analysis showed that open reading frames of the PB2 and PB1 cDNAs contain 2 280 nt and 2 277 nt encoding 759 and 758 amino acids,respectively.Nucleotide sequence and amino acid identity rates of the polymerase genes PB2 and PB1 were over 95% between avian influenza virus strain QA/HK/G1/97 that belong to the subtype H9N2 AIV,there were in closer relation between them. |
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| Keywords: | Avian influenza virus H9N2 polymerase gene sequence analysis |
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