| Establishment of the Determination Methods of Total Anthraquinone Content in Different Processed Products of Rhubarb |
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| 作者姓名： | Jiangcun WEI Xiumei MA Chunli TANG Shaofeng CHEN Yusheng HUANG Bihua NONG Dongmei HUANG Guangli LU |
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| 作者单位： | Guangxi International Zhuang Medicine Hospital;The First Affiliated Hospital of Guangxi University of Chinese Medicine |
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| 基金项目： | Supported by Youth Fund Project of Guangxi University of Chinese Medicine(2019QN036);National Talent Training Program for Inheriting Characteristic Technology of Traditional Chinese Medicine(20184828005);Guangxi Natural Science Foundation Project(2020GXNSFAA259059);The Project of Improving the Basic Scientific Research Ability of Young-and Middle-aged Teachers in Guangxi Universities in 2019(2019KY0341);In Hospital Preparation Development Project of the First Affiliated Hospital of Guangxi University of Chinese Medicine in 2017(2017ZJ001);Opening Project of Guangxi Zhuang Yao Medicine Key Laboratory(GXZYKF2020A-08);Scientific Research Projects of Guangxi International Zhuang Medicine Hospital in 2020(GZ202001). |
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| 摘 要： | Objectives]Ultraviolet spectrophotometry was established for the determination of total content of anthraquinone in different processed products of rhubarb.Methods]Emodin was used as the control substance,and its color was developed by magnesium acetate methanol solution with the concentration of 1%,the absorbance was determined at the wavelength of 516 nm,the content of total anthraquinone in different processed products of rhubarb was determined to compare the content differences.Results]The regression equation was y=0.0452 x+0.0015,R 2=0.9991.Emodin had a good linear range in the concentration of 0.98-23.52μg/mL,with high accuracy,good repeatability and stable detection results.This method revealed the content changes of total anthraquinone in rhubarb before and after processing.Conclusions]This method could be used as the quality control method of different processed products of rhubarb,and provided reference for the comprehensive development and utilization of rhubarb resources in the future.
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| 关 键 词： | RHUBARB Total anthraquinone Ultraviolet spectrophotometry |
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