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| 表达禽流感病毒H5HA、H7HA基因DNA疫苗的免疫原性研究 | |
| 引用本文： | 张芳芳,姜永萍,刘光亮,陈怀涛,乔传玲,陈化兰.表达禽流感病毒H5HA、H7HA基因DNA疫苗的免疫原性研究[J].中国预防兽医学报,2006,28(1):1-5. |
| 作者姓名： | 张芳芳 姜永萍 刘光亮 陈怀涛 乔传玲 陈化兰 |
| 作者单位： | 1. 中国农业科学院哈尔滨兽医研究所,农业部动物流感重点开放实验室,兽医生物技术国家重点实验室,黑龙江,哈尔滨,150001;甘肃农业大学,甘肃,兰州,730070 2. 中国农业科学院哈尔滨兽医研究所,农业部动物流感重点开放实验室,兽医生物技术国家重点实验室,黑龙江,哈尔滨,150001 3. 中国农业科学院哈尔滨兽医研究所,农业部动物流感重点开放实验室,兽医生物技术国家重点实验室,黑龙江,哈尔滨,150001;新疆农业大学,新疆,乌鲁木齐,830052 4. 甘肃农业大学,甘肃,兰州,730070 |
| 摘 要： | 为研究双顺反子DNA疫苗对禽流感病毒（AIV）的保护作用，将H5和H7亚型AIV的HA基因克隆到同一表达栽体上，构建了双顺反子HA基因表达质粒pCI—H5HA—H7HA。以此质粒肌注免疫4周龄SPF鸡，首次免疫后3周加强免疫，同时设pCI—H5HA和pCI—H7HA联合免疫组及空白对照组，每周采血用微量血凝抑制法检测HI抗体。加强免疫后3周分别以100LD50的高致病力禽流感病毒（HPAIV）A／Goose／Guangdong／1／96（H5N1）和A／FPv／Rostock／34〈（H7N1）进行致死性攻击。结果显示各免疫组均可刺激鸡体产生H5、H7特异性抗体，pCI—H5HA—H7HA诱导产生的抗体对H5N1和H7N1的攻毒保护分别为50%和10％，而pCI—H5HA和pCI—H7HA联合免疫的攻毒保护均为70％。表明双顺反子质粒可诱导鸡产生较好或一定的保护，但免疫效果不够理想，推测与DNA的摄取及其体内表达有关，可望通过调整DNA疫苗的多种因素提高免疫效果。 |
| 关 键 词： | 禽流感 H5亚型 H7亚型 HA基因 DNA疫苗 |
| 文章编号： | 1008-0589（2006）01-0001-05 |
| 收稿时间： | 2004-08-12 |
| 修稿时间： | 2004年8月12日 |
The immunogenicity of DNA vaccine co-expressing HA gene of H5 and H7 subtype AIV |
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| ZHANG Fang-fang,JIANG Yong-ping,LIU Guang-liang,CHEN Huai-tao,QIAO Chuan-ling,CHEN Hua-lan.The immunogenicity of DNA vaccine co-expressing HA gene of H5 and H7 subtype AIV[J].Chinese Journal of Preventive Veterinary Medicine,2006,28(1):1-5. | |
| Authors: | ZHANG Fang-fang JIANG Yong-ping LIU Guang-liang CHEN Huai-tao QIAO Chuan-ling CHEN Hua-lan |
| Abstract: | The protective efficacy of a plasmid DNA that contains two AIV HA genes was evaluated in SPF chickens.Two AIV HA genes of H5 and H7 subtype were cloned into the same vector,thus a dou-blegene vector-pCI-H5HA-H7HA had been constructed successfully.And this plasmid was used to immu-nize 4-week age SPF chickens by intramuscular injection.For comparison,another group of SPF chick-ens were vaccinated with pCI-H5HA and pCI-H7HA jointly.The Haemagglutinin inhibition(HI) titer induced by DNA vaccine were tested weekly for 6 weeks and the protection ratio were calculated.The SPF chick-ens were challenged with H5N1 or H7N1 isolate of HPAIV at the sixth week after the immunization.And the result showed,50 % and 10 % protection rate against H5N1 and H7N1 were obtained prospectively from pCI-H5HA-H7HA vaccinated groups,while 70 % of protection rate were obtained from the groups immunized with pCI-H5HA and pCI-H7HA jointly.The study suggested the possibility that the dou-ble-gene plasmid can induce protection more or less,but the titer is yet unsatisfactory,inferring that the ingestion of DNA and its expressionlevel may play an importantrole.In orderto improve the protec-tive efficacy,the further study on various influencing factor is still needed. |
| Keywords: | avian influenza H5 subtype H7 suhtype HA gene DNA vaceine |
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