Availability of genetically modified soybean meal in rainbow trout Oncorhynchus mykiss diets |
| |
| Authors: | Pitchaya Chainark Shuichi Satoh Tokuya Hino Viswanath Kiron Ikuo Hirono Takashi Aoki |
| |
| Institution: | (1) Laboratory of Fish Nutrition, Tokyo University of Marine Science and Technology, 108-8477 Tokyo, Japan;(2) Genome Science, Department of Marine Biosciences, Tokyo University of Marine Science and Technology, 108-8477 Tokyo, Japan |
| |
| Abstract: | The use of genetically modified defatted soybean meal (GM SBM) as rainbow trout feed was investigated, in comparison with
non-GM SBM. Both SBMs were included at levels of approximately 15 and 30% in four diets (42% protein). The diets were fed
to juvenile fish (48.3 g average weight) for 12 weeks. The nutrient use showed that there was no significant difference in
growth and feed performance between GM and non-GM SBM groups at both inclusion levels at the end of 12th week. The cauliflower
mosaic virus 35S promoter fragment (220 bp) of the GM SBM was detected in the muscle of fish receiving both levels of GM SBM diet by nested
PCR, but the frequency of detection was greater at the higher inclusion level. Additionally, the promoter fragment was not
detected by the fifth day after changing the diet to non-GM. Conversely, the promoter fragment was not detected from fish
fed with the non-GM SBM diet. The results demonstrated that the availability of protein in GM SBM was similar to that of non-GM
SBM, and the promoter fragments found in the muscle of fish were not detectable after changing the diet to non-GM, verifying
the availability of the GM SBM in rainbow trout feed. |
| |
| Keywords: | availability cauliflower mosaic virus 35S promoter genetically modified defatted soybean meal use |
| 本文献已被 SpringerLink 等数据库收录！ |
|
