| 利用幼穗愈伤组织筛选小麦抗赤霉病菌毒素体细胞突变体的研究 |
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| 引用本文： | 阎博,薛秀庄,陈建莉,赵会贤,柴冬梅.利用幼穗愈伤组织筛选小麦抗赤霉病菌毒素体细胞突变体的研究[J].西北农业学报,1995,4(3):8-12. |
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| 作者姓名： | 阎博 薛秀庄 陈建莉 赵会贤 柴冬梅 |
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| 作者单位： | 陕西省农业科学院生物技术室, 杨陵712100;陕西省农业科学院生物技术室, 杨陵712100;陕西省农业科学院生物技术室, 杨陵712100;陕西省农业科学院生物技术室, 杨陵712100;陕西省农业科学院生物技术室, 杨陵712100 |
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| 摘 要： | 以不同小麦品种（系）幼穗诱导的愈伤组织进行小麦抗赤霉病体细胞突变体筛选，结果表明，不同赤霉病抗性品种（系）对赤霉菌素表现不同的反应，抗性品种愈伤组织生长受毒素抑制小，感病品种伤组织受毒素抑制强烈，筛选出了赤霉病菌毒素加入的最适浓度为２％（Ｖ／Ｖ），在该浓度下可筛选出抗性突变体，获得了咸农１５１，陕１６７，陕２２９等品种（系）的赤霉病菌素抗性体细胞无性系再生植株，再生植株田间人工接种鉴定表现出中或高
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| 关 键 词： | 小麦 幼穗愈伤组织 赤霉病 突变体 抗病性 |
| 收稿时间： | 1994/8/19 0:00:00 |
| 修稿时间： | 1995/1/13 0:00:00 |
SCREENING SCAB RESISTANT MUTANTS YOUNG INFLORESCENCE CULTURE OF WHEAT |
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| Yan Bo,Xue Xiuzhuang,Chen Jianli,Zhao Huixian and Chai Dongmei.SCREENING SCAB RESISTANT MUTANTS YOUNG INFLORESCENCE CULTURE OF WHEAT[J].Acta Agriculturae Boreali-occidentalis Sinica,1995,4(3):8-12. |
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| Authors: | Yan Bo Xue Xiuzhuang Chen Jianli Zhao Huixian and Chai Dongmei |
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| Institution: | Biotechnology Laboratory, Shaanxi Academy of Agricultural Sciences, Yangling 712100;Biotechnology Laboratory, Shaanxi Academy of Agricultural Sciences, Yangling 712100;Biotechnology Laboratory, Shaanxi Academy of Agricultural Sciences, Yangling 712100;Biotechnology Laboratory, Shaanxi Academy of Agricultural Sciences, Yangling 712100;Biotechnology Laboratory, Shaanxi Academy of Agricultural Sciences, Yangling 712100 |
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| Abstract: | Callus induced from young inflorescence of different wheat varieties were used as objects for wheet selection,crude toxinic extracts from scab pathogen (Fusarium graminearum schwebe) was used as selective inoculum to add to selective medium.Selections of scab resistant mutants of wheat were conducted on differentiation medium.The results showed that different responses were found among varieties with different scab resistant levels,callus derived from sus ceptible varieties were inhibited more greatly by crude toxin than resistant ones.The optimum concentration for addtion of crude toxin is 2%.4 somatic cell mutants of wheat resistant to scab were screemed,they are Shaan 167MR-1, Xiannong 151 MR-1.Xiannong 1518-1 and Shaan 229 MR-1, these regenerated mutant plants showed a high or moderate resistance to wheat scab pathogen when artificially inoculated in the field in 1993.But their resistant stability is worth of further study. |
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| Keywords: | Wheat Inflorescence-derived callus Scab pathotoxin Scab resistant mutant |
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